FFPE human lymph node sections stained with 100 ul anti-AIF1 (clone AIF1/1909) at 1:400. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-AIF1 (clone AIF1/1909) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-AIF1 antibody (Clone AIF1/1909). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Formalin-fixed, paraffin-embedded Human Lymph Node stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/1909).

AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 199.00$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 199-MSM1
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
199-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
199-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
199-MSM1-P1ABX Size: 100 ug, Tag: Unconjugated $ 429.00
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Datasheets and Documentation
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SKU: 199-MSM1-P0
AIF1 / Iba1 (Microglia Marker) General Information
Alternate Names
Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
Molecular Weight
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol199
Entrez Gene IDAIF1
UniProt ID(s)P55008
UniGene ID(s)Hs76364
KEGG Gene ID(s)hsa:199
General Description of AIF1 / Iba1 (Microglia Marker).
AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Human Anti-AIF1 / Iba1 (Microglia Marker) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-AIF1 / Iba1 (Microglia Marker) Antibody Clone: AIF1/1909
Clone AIF1/1909 Host and Isotype: Mouse IgG1, kappa
Anti-Human AIF1 / Iba1 (Microglia Marker) Positive Control Sample: Lymph Node, Tonsil or Kidney
Cellular Localization of Antibody AIF1/1909 Staining: Cytoplasmic and Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Purified fragment of human recombinant AIF1 protein (apprx. aa 1-146) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

AIF1 / Iba1 (Microglia Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, AIF1 / Iba1 (Microglia Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-AIF1 Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/AIF1 IHC Human Kidney (AIF1 1909)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/AIF1 IHC Human Lymph Node (AIF1 1909)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/AIF1 IHC human Tonsil (AIF1 1909)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-AIF1 (AIF1 1909) Gel-210x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-199-MSM1-P0-anti-AIF1-Iba1-Microglia-Marker-antibody-1-300x225.jpeg