FFPE human pancreas sections stained with 100 ul anti-AKT1 (clone AKT1/2552) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-AKT1 antibody (Clone AKT1/2552). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing more than 19,000 full-length human proteins using AKT1 Mouse Monoclonal Antibody (AKT1/2552).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

AKT1 (Prognostic Marker for Neuroendocrine Tumors) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 207-MSM2
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
207-MSM2-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
207-MSM2-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
207-MSM2-P1ABX Size: 100 ug, Tag: Unconjugated $ 488.00
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SKU: 207-MSM2-P0
AKT1 (Prognostic Marker for Neuroendocrine Tumors) General Information
Alternate Names
AKT1; Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha
Molecular Weight
62kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol207
Entrez Gene IDAKT1
UniProt ID(s)P31749
UniGene ID(s)Hs525622
COSMIC ID Link(s)AKT1
KEGG Gene ID(s)hsa:207
General Description of AKT1 (Prognostic Marker for Neuroendocrine Tumors).
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Human Anti-AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody Clone: AKT1/2552
Clone AKT1/2552 Host and Isotype: Mouse IgG2b, kappa
Anti-Human AKT1 (Prognostic Marker for Neuroendocrine Tumors) Positive Control Sample: PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.
Cellular Localization of Antibody AKT1/2552 Staining: Nuclear, Cytoplasmic and Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment of human AKT1 protein (apprx. aa 85-189) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

AKT1 (Prognostic Marker for Neuroendocrine Tumors) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, AKT1 (Prognostic Marker for Neuroendocrine Tumors) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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AKT1 (Prognostic Marker for Neuroendocrine Tumors) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-AKT1 Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/AKT1 IHC human Pancreas (AKT1 2552)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-AKT1 (AKT1 2552) Gel.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-AKT1 Western HeLa (AKT1 2552)-189x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-207-MSM2-P0-anti-AKT1-Prognostic-Marker-for-Neuroendocrine-Tumors-antibody-1-300x120.jpeg