Formalin-fixed, paraffin embedded human testis sections stained with 100 ul anti-MAGE-1 (clone MA454) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Formalin-fixed, paraffin-embedded human Testis stained with MAGE-1 Monoclonal Antibody (MA454).

Anti-MAGE-1 Antibody Clone MA454

Name: Anti-MAGE-1 Antibody Clone MA454
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 4100-MSM1
Species: Human, Rat, and Canine
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
4100-MSM1-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 4100-MSM1-P1

MAGE-1 General Information
Alternate Names
Melanoma-associated antigen 1, MAGEA1
Molecular Weight
42-46kDa
Chromosomal Location
Xq28
Curated Database and Bioinformatic Data
Gene Symbol	MAGEA1
Entrez Gene ID	4100
Ensemble Gene ID	ENSG00000198681
RefSeq Protein Accession(s)	NP_004979
RefSeq mRNA Accession(s)	NM_004988,
RefSeq Genomic Accession(s)	NG_013243, NC_018934, NC_000023
UniProt ID(s)	P43355
UniGene ID(s)	P43355
HGNC ID(s)	6796
Cosmic ID(s)	MAGEA1
KEGG Gene ID(s)	hsa:4100
PharmGKB ID(s)	PA30542
General Description of MAGE-1.
Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed, specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas, primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas), not on normal tissues, the encoded antigen may serve as a marker of early detection, target for cancer immunotherapy.

Human, Rat, and Canine Anti-MAGE-1 Antibody Product Attributes

Species: Human, Rat, and Canine

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-MAGE-1 Antibody Clone: MA454

Clone MA454 Host and Isotype: Mouse IgG1 kappa

Anti-Human, Rat, and Canine MAGE-1 Positive Control Sample: Melanoma cell lines. Melanomas or testicular carcinomas.

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Human MAGE-A1 full length recombinant protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

MAGE-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MAGEA1 antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in MAGE-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis. Low, but measureable presence of MAGE-1 could be seen in. We were unable to detect MAGE-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's MAGE-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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