Staining with mouse monoclonal ACTH [Clone 2F6] antibody in formalin-fixed paraffin-embedded human pituitary gland.
Staining with mouse monoclonal ACTH [Clone 2F6] antibody in formalin-fixed paraffin-embedded human pituitary gland.

Anti-ACTH Antibody Clone 2F6

$ 298.00
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SKU: 5443-MSM4
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5443-MSM4-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 5443-MSM4-P1
ACTH General Information
Alternate Names
Pro-opiomelanocortin, POMC
Molecular Weight
ACTH is ~5kDa,, the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation, post-translational modifications.
Chromosomal Location
2p23.3
Curated Database and Bioinformatic Data
Gene SymbolPOMC
Entrez Gene ID5443
Ensemble Gene IDENSG00000115138
RefSeq Protein Accession(s)NP_001306134, NP_000930, NP_001030333, NP_001306133
RefSeq mRNA Accession(s)NM_001319204 NM_001035256, NM_001319205, NM_000939
RefSeq Genomic Accession(s)NG_008997, NC_000002, NC_018913
UniProt ID(s)P01189
UniGene ID(s)P01189
HGNC ID(s)9201
Cosmic ID(s)POMC
KEGG Gene ID(s)hsa:5443
PharmGKB ID(s)PA33526
General Description of ACTH.
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin, Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells,, macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors, the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Human, Mouse, and Rat Anti-ACTH Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-ACTH Antibody Clone: 2F6
Clone 2F6 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat ACTH Positive Control Sample: Normal pituitary gland or pituitary tumor
Cellular Localization of Antibody Cytoplasm, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

ACTH Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in ACTH antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the anterior in pituitary gland. More moderate antibody staining intensity was present in cells in the anterior in pituitary gland. Low, but measureable presence of ACTH could be seen in. We were unable to detect ACTH in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's ACTH Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-ACTH Antibody Clone 2F6

https://enquirebio.com/wp-content/uploads/2019/01/ACTH IHC human Pituitary Gland (2F6)-300x225.jpg