FFPE human pituitary gland sections stained with 100 ul anti-ACTH (clone r57) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
FFPE human pituitary gland sections stained with 100 ul anti-ACTH (clone r57) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-ACTH antibody (Clone r57). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Formalin-fixed, paraffin-embedded human Pituitary Gland stained with ACTH Recombinant Mouse Monoclonal Antibody (r57).

Anti-ACTH Antibody Clone r57

$ 228.00$ 518.00
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SKU: 5443-MSM6
Species: Human, Mouse, and Rat
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5443-MSM6-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
5443-MSM6-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
5443-MSM6-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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SKU: 5443-MSM6-P0
ACTH General Information
Alternate Names
Pro-opiomelanocortin, POMC
Molecular Weight
ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolPOMC
Entrez Gene ID5443
Ensemble Gene IDENSG00000115138
RefSeq Protein Accession(s)NP_001306134, NP_000930, NP_001030333, NP_001306133
RefSeq mRNA Accession(s)NM_001319204 NM_001035256, NM_001319205, NM_000939
RefSeq Genomic Accession(s)NG_008997, NC_000002, NC_018913
UniProt ID(s)P01189
UniGene ID(s)P01189
HGNC ID(s)9201
Cosmic ID(s)POMC
KEGG Gene ID(s)hsa:5443
PharmGKB ID(s)PA33526
General Description of ACTH.
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Human, Mouse, and Rat Anti-ACTH Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Immunohistochemistry (IHC).
Clonality: Recombinant Monoclonal
Anti-ACTH Antibody Clone: r57
Clone r57 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat ACTH Positive Control Sample: Normal pituitary gland or pituitary tumor.
Cellular Localization of Antibody Cytoplasm and Secreted
Buffer and Stabilizer: 10mM PBS with or without 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: N-terminal fragment of human ACTH conjugated to KLH
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

ACTH Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in ACTH antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the anterior in pituitary gland. More moderate antibody staining intensity was present in cells in the anterior in pituitary gland. Low, but measureable presence of ACTH could be seen in. We were unable to detect ACTH in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's ACTH Recombinant Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-ACTH Antibody Clone r57

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