Immunohistochemical staining of ACTH  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

ACTH [AH26] Antibody (cGMP).

$ 128.00$ 588.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD74
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD74-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD74-0.5ml Size: 0.5 ml, Format: Concentrate $ 348.00
QIVD74-1ml Size: 1 ml, Format: Concentrate $ 588.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD74-0.1ml
ACTH General Information
Alternate Names
Molecular Weight
29.4 kDa
Chromosomal Location
p23.3 [chr: 2] [chr_start: 25160853] [chr_end: 25168903] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPOMC
Entrez Gene ID5443
RefSeq Protein Accession(s)NP_001030333; NP_000930; NP_001306134; NP_001306133
RefSeq mRNA Accession(s)NM_001319204; NM_001035256; NM_001319205; NM_000939
RefSeq Genomic Accession(s)NG_008997; NC_000002
UniProt ID(s)P01189
PharmGKB ID(s)PA33526
KEGG Gene ID(s)hsa:5443
Associated Diseases (KEGG IDs)Obesity (OBESITY) [MIM:601665]: A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. {ECO:0000269|PubMed:12165561}. Disease susceptibility may be associated with variations affecting the gene represented in this entry.; Obesity, early-onset, with adrenal insufficiency and red hair (OBAIRH) [MIM:609734]: An autosomal recessive disorder characterized by early-onset obesity due to severe hyperphagia, pigmentary abnormalities, mainly pale skin and red hair, and secondary hypocortisolism. {ECO:0000269|PubMed:9620771}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of ACTH .
This antibody is specific to an epitope on the N-terminus of ACTH. This antibody stains corticotrophs in the adenohypophysis and is useful in the classification of pituitary adenomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-ACTH Antibody Clone: AH26
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Pituitary
Cellular Localization of Antibody AH26 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a synthetic peptide corresponding to amino acids 1-24 from the N-terminal of human ACTH.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

ACTH Information for Pathologists

Summary:

Adrenocorticotrophic hormone (ACTH) is a peptide hormone secreted from the anterior pituitary gland that stimulates the adrenal cortex to secrete glucocorticoid hormones. The hormone comprises a long chain of amino acids that share a high degree of homology between humans, cows, pigs and sheep. Mature ACTH is derived from POMC cleavage in response to CRH release from the hypothalamus.

Common Uses By Pathologists:

ACTH immunostaining is useful for classifying pituitary tumors and other pituitary disease. It reacts with corticotrophs (cells that produce ACTH) including some small-cell carcinomas present in lung tissue which cause Paraneoplastic Syndromes.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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ACTH [AH26] Antibody (cGMP).