Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:75 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Actin, Muscle Antibody Clone: HHF35
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Skeletal muscle
Cellular Localization of Antibody HHF35 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with SDS extracted protein from human myocardium.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Actin, Muscle Information for Pathologists
Summary:
Discovered in 1987 (Am J Pathol 1987;126:51); also called HHF35, MSA. Recognizes all alpha actins (skeletal, smooth, cardiac) and gamma smooth muscle actin; but not beta cytoplasmic or gamma cytoplasmic actin (the latter is also called non-muscle actin). Recognizes actin expressed in all cells with muscle differentiation (cardiac, smooth and skeletal muscle), myoepithelial cells, myofibroblasts, pericytes and myogenic tumors (Am J Clin Pathol 1991;96:32). Uses by pathologists Identify skeletal muscle (Tumori 2007;93:198, J Cutan Pathol 2007;34:352) and smooth muscle cells (Eur Respir J 2001;17:316) in normal tissue or various disease entities.Common Uses By Pathologists:
Identify skeletal muscle (Tumori 2007;93:198, J Cutan Pathol 2007;34:352) and smooth muscle cells (Eur Respir J 2001;17:316) in normal tissue or various disease entities. Classify tumors with smooth or skeletal muscle, pericytes, myofibroblasts (Cardiovasc Pathol 2006;15:91) or myoepithelial cells. Differentiate leiomyosarcoma (MSA+, keratin-) from spindle cell carcinoma (MSA-, keratin+, Am J Otolaryngol 2005;26:201).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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