Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)Western Blot of hSKM Cell Lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Anti-Actin, Muscle Specific Antibody Clone MSA/953

$ 329.00
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SKU: MSM2-953
Species: Human, Rabbit, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: MSM2-953-P1
Actin, Muscle Specific General Information
Alternate Names
ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1, anti-ACTA antibody, anti-ACTA1 antibody, anti-ACTA2 antibody, anti-ACTC1 antibody, anti-Actin antibody, anti-ACTSA antibody, anti-Alpha-2 actin antibody, anti-alpha skeletal muscle antibody, anti-Alpha-actin-1 antibody, anti-Cardiac muscle alpha actin-1 antibody, anti-Skeletal muscle alpha actin-1 antibody
Molecular Weight
Multiple
Chromosomal Location
1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Curated Database and Bioinformatic Data
Gene SymbolACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth)
Entrez Gene ID58, 59, 70
Ensemble Gene IDENSG00000143632, ENSG00000107796, ENSG00000159251
RefSeq Protein Accession(s)NP_001091, NP_001135417, NP_001604, NP_001307784, NP_005150
RefSeq mRNA Accession(s)NM_001100, NM_001141945 NM_001613, NM_001320855NM_005159
RefSeq Genomic Accession(s)NG_006672, NC_018912, NC_000001, NC_000010, NC_018921, NG_011541, NG_007553, NC_018926, NC_000015
UniProt ID(s)P68133, P02568, P99020, P03996, P04108, D2JYH4, P62736, P68032, B3KPP5
UniGene ID(s)P68133, P02568, P99020, P03996, P04108, D2JYH4, P62736, P68032, B3KPP5
HGNC ID(s)129, 130, 143
Cosmic ID(s)ACTA1, ACTA2, ACTC1
KEGG Gene ID(s)hsa:58, hsa:59, hsa:70
PharmGKB ID(s)PA24455, PA24456, PA162375571
General Description of Actin, Muscle Specific.
This antibody recognizes actin of skeletal, cardiac,, smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta,, gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha, gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells, connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas,, lymphomas are negative.It stains tumors of smooth muscle (leiomyomas, leiomyosarcomas) as well as skeletal muscle (rhabdomyomas, rhabdomyosarcomas).

Human, Rabbit, and Rat Anti-Actin, Muscle Specific Antibody Product Attributes

Species: Human, Rabbit, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Actin, Muscle Specific Antibody Clone: MSA/953
Clone MSA/953 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rabbit, and Rat Actin, Muscle Specific Positive Control Sample: Muscle or sarcoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Synthetic peptide common among human muscle actins
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Actin, Muscle Specific Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's Actin, Muscle Specific Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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