Immunohistochemical staining of Actin, Sarcomeric  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Actin, Sarcomeric [5C5] Antibody (cGMP).

$ 135.00$ 555.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgM

Product NumberDescriptionPrice
QIVD222-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD222-0.5ml Size: 0.5 ml, Format: Concentrate $ 378.00
QIVD222-1ml Size: 1 ml, Format: Concentrate $ 628.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD222-0.1ml
Actin, Sarcomeric General Information
Alternate Names
Molecular Weight
107 kDa
Chromosomal Location
2C7 [chr: X] [chr_start: 2023822] [chr_end: 2042311] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolActn
Entrez Gene ID31166
RefSeq Protein Accession(s)NP_001188533; NP_001188532; NP_001259165; NP_477484; NP_477485; NP_001259167; NP_001259166; NP_726784
RefSeq mRNA Accession(s)NM_001272238; NM_166920; NM_001201604; NM_058136; NM_001272236; NM_001272237; NM_001201603; NM_001272235; NM_058137
RefSeq Genomic Accession(s)NC_004354
UniProt ID(s)P18091
KEGG Gene ID(s)dme:Dmel_CG4376
General Description of Actin, Sarcomeric .
Monoclonal anti-alpha-sarcomeric actin has been used as a marker for rhabdomyosarcoma. This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It does not react with smooth muscle tissue.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at RoomTemperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Actin, Sarcomeric Antibody Clone: 5C5
Host and Isotype: Mouse IgM
Recommended Positive Control Sample: Skeletal muscle
Cellular Localization of Antibody 5C5 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with purified rabbit striated muscle actin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Actin, Sarcomeric Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Actin, Sarcomeric [5C5] Antibody (cGMP).