Formalin-fixed, paraffin embedded rat lung sections stained with 100 ul anti-Smooth Muscle Actin (clone ACTA2/791) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).

Anti-Actin, Smooth Muscle Antibody Clone ACTA2/791

$ 429.00
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SKU: 59-MSM2
Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
59-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 59-MSM2-P1
Actin, Smooth Muscle General Information
Alternate Names
Alpha-actin-2, actinaortic smooth muscle, alpha smooth muscle actin, ASMA, ACTA2
Molecular Weight
42kDa
Chromosomal Location
10q23.31
Curated Database and Bioinformatic Data
Gene SymbolACTA2
Entrez Gene ID59
Ensemble Gene IDENSG00000107796
RefSeq Protein Accession(s)NP_001135417, NP_001604, NP_001307784
RefSeq mRNA Accession(s)NM_001141945 NM_001613, NM_001320855
RefSeq Genomic Accession(s)NC_000010, NC_018921, NG_011541
UniProt ID(s)P03996, P04108, D2JYH4, P62736
UniGene ID(s)P03996, P04108, D2JYH4, P62736
HGNC ID(s)130
Cosmic ID(s)ACTA2
KEGG Gene ID(s)hsa:59
PharmGKB ID(s)PA24456
General Description of Actin, Smooth Muscle.
Actin is a major component of the cytoskeleton, is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts, myoepithelial cells. This antibody could be used together with anti-muscle specific actin, myogenin in making a diagnosis of smooth muscle, skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin, myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin, anti-smooth muscle actin, are negative with anti-myogenin.

Human, Rat, and Broad Species Reactivity Anti-Actin, Smooth Muscle Antibody Product Attributes

Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Actin, Smooth Muscle Antibody Clone: ACTA2/791
Clone ACTA2/791 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Rat, and Broad Species Reactivity Actin, Smooth Muscle Positive Control Sample: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ACTA2 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Actin, Smooth Muscle Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Actin, Smooth Muscle antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. Low, but measureable presence of Actin, Smooth Muscle could be seen in. We were unable to detect Actin, Smooth Muscle in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Actin, Smooth Muscle expression as measured by anti-Actin, Smooth Muscle antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - + - - - - ++ - - - -
ACTA2 Variability ++ + ++ ++ + + + + ++ ++ ++ + + + + +++ + + ++ +

Limitations and Warranty

enQuire Bio's Actin, Smooth Muscle Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Actin, Smooth Muscle Antibody Clone ACTA2/791

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