Immunohistochemical staining of Actin, Smooth Muscle  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

DISC: Actin, Smooth Muscle [1A4] Antibody

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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a, kappa

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Datasheets and Documentation
Product Datasheet
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Bioactivity (test results eg. IU/ml):

SKU: QIVD94-0.1ml
Actin, Smooth Muscle General Information
Alternate Names
Molecular Weight
42 kDa
Chromosomal Location
q23.31 [chr: 10] [chr_start: 88935074] [chr_end: 88991339] [strand: -1]
Curated Database and Bioinformatic Data
Gene Symbol ACTA2
Entrez Gene ID 59
RefSeq Protein Accession(s) NP_001604; NP_001307784; NP_001135417
RefSeq mRNA Accession(s) NM_001141945; NM_001613; NM_001320855
RefSeq Genomic Accession(s) NC_000010; NG_011541
UniProt ID(s) P62736
PharmGKB ID(s) PA24456
KEGG Gene ID(s) hsa:59
Associated Diseases (KEGG IDs) ACTA2 mutations predispose patients to a variety of diffuse and diverse vascular diseases, premature onset coronary artery disease (CAD), premature ischemic strokes and Moyamoya disease. {ECO:0000269|PubMed:19409525}.; Aortic aneurysm, familial thoracic 6 (AAT6) [MIM:611788]: A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. {ECO:0000269|PubMed:17994018, ECO:0000269|PubMed:19409525, ECO:0000269|PubMed:19639654}. The disease is caused by mutations affecting the gene represented in this entry.; Moyamoya disease 5 (MYMY5) [MIM:614042]: A progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia. The abnormal vessels resemble a 'puff of smoke' (moyamoya) on cerebral angiogram. Affected individuals can develop transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage. Hemiplegia of sudden onset and epileptic seizures constitute the prevailing presentation in childhood, while subarachnoid bleeding occurs more frequently in adults. {ECO:0000269|PubMed:20970362}. The disease is caused by mutations affecting the gene represented in this entry.; Multisystemic smooth muscle dysfunction syndrome (MSMDYS) [MIM:613834]: A syndrome characterized by dysfunction of smooth muscle cells throughout the body, leading to aortic and cerebrovascular disease, fixed dilated pupils, hypotonic bladder, malrotation, and hypoperistalsis of the gut and pulmonary hypertension. {ECO:0000269|PubMed:20734336}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Actin, Smooth Muscle .
This antibody is specific to alpha-smooth muscle isoform of actin. It reacts with smooth muscle cells of vessels and different parenchymes. This antibody does not cross-react with beta and γ-cytoplasmic, alpha-sarcomeric and alpha-myocardial actin isoforms.


Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Actin, Smooth Muscle Antibody Clone: 1A4
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Leiomyoma, Colon
Cellular Localization of Antibody 1A4 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Actin, Smooth Muscle Information for Pathologists


Discovered in 1986 (J Cell Biol 1986;103:2787). Antibodies to alpha smooth muscle actin do not detect the other actin isoforms. Terminology Also called smooth muscle actin, SMA; clone 1A4 or sm-1. Clinical features

Notable Clinical Features:

No apparent deficiency in intestinal pseudoobstruction (J Clin Pathol 2004;57:1168). May predict aggressive behavior in cutaneous basal cell carcinoma (Hum Pathol 2010;41:1128) and pancreatic ductal adenocarcinoma (Pancreas 2010 May 7 [Epub ahead of print]). Predictor of good prognosis in lung adenocarcinoma (Mod Pathol 2009;22:776). A potential prognostic factor in idiopathic pulmonary fibrosis (Clinics (Sao Paulo) 2012;67:1039). Interpretation

Common Uses By Pathologists:

Identify smooth muscle cells and myofibroblasts in normal, reactive (Am J Respir Cell Mol Biol 1999;20:582) or neoplastic tissue (Am J Dermatopathol 2006;28:105). Identify myoepithelial cells in normal, neoplastic or diseased breast, salivary glands or sweat glands; may be helpful to rule out invasion; may be particularly important in cytology specimens (Anticancer Res 2003;23:4175). Identify pericytes to correlate with hematogenous metastasis and prognosis (Oncology 2005;69:159). Help distinguish pleuropulmonary desmoid tumors (SMA+) from solitary fibrous tumor (SMA-, Arch Pathol Lab Med 2006;130:1503). Note: in breast papillary lesions, p63 is more sensitive and specific because smooth muscle actin also stains stromal cells (J Clin Pathol 2007;60:315).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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DISC: Actin, Smooth Muscle [1A4] Antibody