Staining by anti-Adipophilin / Perilipin-2 Antibody 1

Anti-Adipophilin / Perilipin-2 Antibody Clone ADFP/1493

$ 118.00$ 418.00
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SKU: 123-MSM3
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b lambda
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 123-MSM3-P0
Adipophilin / Perilipin-2 General Information
Alternate Names
Lipoprotein-associated phospholipase A2, Lp-PLA2, platelet-activating factor acetylhydrolase, PAF-AH, PLA2G7
Molecular Weight
48kDa
Chromosomal Location
9p22.1
Curated Database and Bioinformatic Data
Gene SymbolPLIN2
Entrez Gene ID123
Ensemble Gene IDENSG00000147872
RefSeq Protein Accession(s)NP_001113, XP_016869748
RefSeq mRNA Accession(s)NR_038064, XM_017014259, NM_001122
RefSeq Genomic Accession(s)NC_000009, NC_018920
UniProt ID(s)Q6FHZ7, Q99541
UniGene ID(s)Q6FHZ7, Q99541
HGNC ID(s)248
Cosmic ID(s)PLIN2
KEGG Gene ID(s)hsa:123
PharmGKB ID(s)PA24569
General Description of Adipophilin / Perilipin-2.
Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Human Anti-Adipophilin / Perilipin-2 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Adipophilin / Perilipin-2 Antibody Clone: ADFP/1493
Clone ADFP/1493 Host and Isotype: Mouse IgG2b lambda
Anti-Human Adipophilin / Perilipin-2 Positive Control Sample: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Adipophilin protein fragment (aa249-376)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Adipophilin / Perilipin-2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-PLIN2 antibody staining is expected to be primarily localized to the lipid droplets.

Observed Antibody Staining Data By Tissue Type:

Variations in Adipophilin / Perilipin-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland, lactating glandular cells in the lactating breast, trophoblastic cells in the placenta and glandular cells in the small intestine. More moderate antibody staining intensity was present in glandular cells in the adrenal gland, lactating glandular cells in the lactating breast, trophoblastic cells in the placenta and glandular cells in the small intestine. Low, but measureable presence of Adipophilin / Perilipin-2 could be seen inrespiratory epithelial cells in the bronchus, endothelial cells in the cerebral cortex, glandular cells in the endometrium and epididymis, cells in the tubules in kidney, hepatocytes in liver, glandular cells in the rectum and stomach and Leydig cells in the testis. We were unable to detect Adipophilin / Perilipin-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Adipophilin / Perilipin-2 expression as measured by anti-Adipophilin / Perilipin-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - ++ - - - ++ - - + - - - +++ - - ++ + ++
PLIN2 Variability ++ ++ ++ ++ ++ ++ + ++ ++ + +++ ++ + ++ ++ ++ ++ +++ ++ +++

Limitations and Warranty

enQuire Bio's Adipophilin / Perilipin-2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Adipophilin / Perilipin-2 Antibody Clone ADFP/1493