Formalin-fixed, paraffin-embedded human Fetal Liver stained with Anti-AFP Monoclonal Antibody (C3). Epitope retrieval (HIER) was performed using citrate buffer pH 6.0. Primary antibody was diluted 1:200 for staining.

Anti-AFP Antibody Clone C3

$ 488.00
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SKU: 174-MSM1
Species: Human, Monkey, Canine, Pig, Cow (-), Canine (-), Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
174-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 174-MSM1-P1
AFP General Information
Alternate Names
Alpha-fetoprotein, AFP, ?-fetoprotein
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolAFP
Entrez Gene ID174
Ensemble Gene IDENSG00000081051
RefSeq Protein Accession(s)NP_001125, NP_001341646
RefSeq mRNA Accession(s)NM_001134, NM_001354717
RefSeq Genomic Accession(s)NC_000004, NG_023028, NC_018915
UniProt ID(s)P02771
UniGene ID(s)P02771
HGNC ID(s)317
Cosmic ID(s)AFP
KEGG Gene ID(s)hsa:174
PharmGKB ID(s)PA24614
General Description of AFP.
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP, shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract,, yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC), germ cell neoplasms, especially yolk sac tumors.

Human, Monkey, Canine, Pig, Cow (-), Canine (-), Mouse (-), and Rat (-) Anti-AFP Antibody Product Attributes

Species: Human, Monkey, Canine, Pig, Cow (-), Canine (-), Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-AFP Antibody Clone: C3
Clone C3 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Canine, Pig, Cow (-), Canine (-), Mouse (-), and Rat (-) AFP Positive Control Sample: Hep-G2 cells. Fetal liver or hepatocellular carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

AFP Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-AFP antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in AFP antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of AFP could be seen inglandular cells in the prostate, hepatocytes in liver and trophoblastic cells in the placenta. We were unable to detect AFP in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of AFP expression as measured by anti-AFP antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + + + + + + ++ + + + ++ + + + + + ++ + +
AFP Variability + ++ + + ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ + + ++ + ++

Limitations and Warranty

enQuire Bio's AFP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-AFP Antibody Clone C3