Western Blot Analysis (A) Recombinant Protein (B) HepG2 Cell lysate Using ALK-1 Monoclonal Antibody (ALK/153).
Western Blot Analysis (A) Recombinant Protein (B) HepG2 Cell lysate Using ALK-1 Monoclonal Antibody (ALK/153).

Anti-ALK Antibody Clone ALK/1503

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 238-MSM3
Species: Human
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 238-MSM3-P0
ALK General Information
Alternate Names
Anaplastic lymphoma kinase, ALK, ALK tyrosine kinase receptor, CD246, Cluster of Differentiation 246, ALK receptor tyrosine kinase
Molecular Weight
80kDa (hybrid) ;200kDa (wild type)
Chromosomal Location
2p23
Curated Database and Bioinformatic Data
Gene SymbolALK
Entrez Gene ID238
Ensemble Gene IDENSG00000171094
RefSeq Protein Accession(s)NP_001340694, NP_004295
RefSeq mRNA Accession(s)NM_001353765 XR_001738688, NM_004304
RefSeq Genomic Accession(s)NC_018913, NC_000002, NG_009445
UniProt ID(s)B6D4Y2, Q9UM73
UniGene ID(s)B6D4Y2, Q9UM73
HGNC ID(s)427
Cosmic ID(s)ALK
KEGG Gene ID(s)hsa:238
PharmGKB ID(s)PA24719
General Description of ALK.
The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Human Anti-ALK Antibody Product Attributes

Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-ALK Antibody Clone: ALK/1503
Clone ALK/1503 Host and Isotype: Mouse IgG1 kappa
Anti-Human ALK Positive Control Sample: Anaplastic Large Cell Lymphoma
Cellular Localization of Antibody Cytoplasmic and nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human ALK protein fragment (aa1360-1460)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

ALK Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ALK antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in ALK antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the cerebral cortex and hippocampus and Purkinje cells in the cerebellum. More moderate antibody staining intensity was present in neuronal cells in the cerebral cortex and hippocampus and Purkinje cells in the cerebellum. Low, but measureable presence of ALK could be seen in cells in the endometrial stroma in endometrium, cells in the molecular layer in cerebellum, decidual cells in the placenta, endothelial cells in the cerebral cortex, epidermal cells in the skin, fibroblasts in mesenchymal tissue, germinal center cells in the lymph node, glandular cells in the adrenal gland, colon, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, rectum, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, Langerhans in skin, Leydig cells in the testis, macrophages in lung, myocytes in heart muscle and skeletal muscle, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, oral mucosa and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. We were unable to detect ALK in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of ALK expression as measured by anti-ALK antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - + + + + + + - + - + ++ - + + + + +
ALK Variability + ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ + ++ ++ ++ + ++ + ++

Limitations and Warranty

enQuire Bio's ALK Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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