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Anti-Alkaline Phosphatase Antibody Clone ALPI/597

$ 298.00
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SKU: 248-MSM2
Species: Human and Cow
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 248-MSM2-P1
Alkaline Phosphatase General Information
Alternate Names
Alkaline phosphatase, Intestinal ALPI, ALPI
Molecular Weight
55kDa
Chromosomal Location
2q37.1
Curated Database and Bioinformatic Data
Gene SymbolALPI
Entrez Gene ID248
Ensemble Gene IDENSG00000163295
RefSeq Protein Accession(s)NP_001622
RefSeq mRNA Accession(s)NM_001631
RefSeq Genomic Accession(s)NC_000002, NC_018913
UniProt ID(s)P09923, A0A024R4A2
UniGene ID(s)P09923, A0A024R4A2
HGNC ID(s)437
Cosmic ID(s)ALPI
KEGG Gene ID(s)hsa:248
PharmGKB ID(s)PA24728
General Description of Alkaline Phosphatase.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like,, liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue, is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia, includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset, severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Human Anti-Alkaline Phosphatase Antibody Product Attributes

Species: Human.
Predicted Cross Reactivity: Bovine.
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (5-10ul/million cells in 0.1ml), Immunofluorescence (1:50-1:100), Immunohistochemistry (IHC) (Formalin-fixed) (1:50-1:100 for 30 min at RT)
Clonality: Monoclonal
Anti-Alkaline Phosphatase Antibody Clone: ALPI/597
Clone ALPI/597 Host and Isotype: Mouse IgG1 kappa
Anti-Human Alkaline Phosphatase Positive Control Sample: Intestine
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human ALP1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Alkaline Phosphatase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ALPI antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Alkaline Phosphatase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the duodenum, small intestine. More moderate antibody staining intensity was present in glandular cells in the duodenum, small intestine. Low, but measureable presence of Alkaline Phosphatase could be seen in. We were unable to detect Alkaline Phosphatase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Alkaline Phosphatase expression as measured by anti-Alkaline Phosphatase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
ALPI Variability + + + + ++ + + + + + + ++ + + + + + ++ ++ +

Limitations and Warranty

enQuire Bio's Alkaline Phosphatase Anti-Human, Cow Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Alkaline Phosphatase Antibody Clone ALPI/597