Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

Anti-Alkaline Phosphatase Antibody Clone ALPL/597

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 249-MSM1
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 249-MSM1-P0
Alkaline Phosphatase General Information
Alternate Names
Alkaline phosphatase, tissue-nonspecific, ALPL, Tissue Nonspecific Alk Phos.
Molecular Weight
55kDa
Chromosomal Location
1p36.12
Curated Database and Bioinformatic Data
Gene SymbolALPL
Entrez Gene ID249
Ensemble Gene IDENSG00000162551
RefSeq Protein Accession(s)XP_005245875, NP_000469, NP_001120973, NP_001170991, XP_006710609, XP_016856392
RefSeq mRNA Accession(s)NM_000478, XM_005245818, XM_006710546, XM_017000903 NM_001177520, NM_001127501
RefSeq Genomic Accession(s)NG_008940, NC_018912, NC_000001
UniProt ID(s)P05186, A0A024RAG0
UniGene ID(s)P05186, A0A024RAG0
HGNC ID(s)438
Cosmic ID(s)ALPL
KEGG Gene ID(s)hsa:249
PharmGKB ID(s)PA24729
General Description of Alkaline Phosphatase.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization ;however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Human Anti-Alkaline Phosphatase Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Alkaline Phosphatase Antibody Clone: ALPL/597
Clone ALPL/597 Host and Isotype: Mouse IgG1 kappa
Anti-Human Alkaline Phosphatase Positive Control Sample: Intestine
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human ALPL protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Alkaline Phosphatase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ALPL antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Alkaline Phosphatase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland. More moderate antibody staining intensity was present in glandular cells in the adrenal gland. Low, but measureable presence of Alkaline Phosphatase could be seen in cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, decidual cells in the placenta, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, small intestine, stomach and thyroid gland, islets of Langerhans in pancreas, keratinocytes in skin, Leydig cells in the testis, myocytes in skeletal muscle, neuronal cells in the caudate nucleus and hippocampus, peripheral nerve/ganglion in colon and urothelial cells in the urinary bladder. We were unable to detect Alkaline Phosphatase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Alkaline Phosphatase expression as measured by anti-Alkaline Phosphatase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - + - - - - - - - - - - - - ++ - -
ALPL Variability + ++ + + ++ ++ + ++ + ++ + ++ + + + + + +++ + ++

Limitations and Warranty

enQuire Bio's Alkaline Phosphatase Monoclonal Antibody is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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