Alpha-1-Antichymotrypsin Validation Dataset

Anti-Alpha-1-Antichymotrypsin Antibody Clone AACT/1451 + AACT/1452

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SKU: 12-MSM3
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 + IgG1
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 12-MSM3-P0
Alpha-1-Antichymotrypsin General Information
Alternate Names
Alpha 1-antichymotrypsin, alpha1AC, a1AC, ?1AC
Molecular Weight
65-76kDa
Chromosomal Location
14q32.1
Curated Database and Bioinformatic Data
Gene SymbolSERPINA3
Entrez Gene ID12
Ensemble Gene IDENSG00000196136
RefSeq Protein Accession(s)NP_001076
RefSeq mRNA Accession(s)NM_001085
RefSeq Genomic Accession(s)NG_012879, NC_000014, NC_018925
UniProt ID(s)P01011, A0A024R6P0
UniGene ID(s)P01011, A0A024R6P0
HGNC ID(s)16
Cosmic ID(s)SERPINA3
KEGG Gene ID(s)hsa:12
PharmGKB ID(s)PA35020
General Description of Alpha-1-Antichymotrypsin.
This MAb recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of ? 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants ;their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Human Anti-Alpha-1-Antichymotrypsin Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Alpha-1-Antichymotrypsin Antibody Clone: AACT/1451 + AACT/1452
Clone AACT/1451 + AACT/1452 Host and Isotype: Mouse IgG1 + IgG1
Anti-Human Alpha-1-Antichymotrypsin Positive Control Sample: HeLa Cells. Tonsil, Pancreas or Histiocytoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Antichymotrypsin (AACT) protein fragment (aa49-187)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Alpha-1-Antichymotrypsin Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Alpha-1-Antichymotrypsin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Alpha-1-Antichymotrypsin could be seen inglandular cells in the adrenal gland, breast, cervix, uterine and epididymis, cells in the tubules in kidney, hepatocytes in liver, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland and seminal vesicle, cells in the seminiferous ducts in testis, Leydig cells in the testis, urothelial cells in the urinary bladder, hematopoietic cells in the bone marrow, glandular cells in the breast, glial cells in the cerebral cortex, glandular cells in the cervix, uterine and gallbladder, myocytes in heart muscle, cells in the tubules in kidney, hepatocytes in liver, germinal center cells in the lymph node, non-germinal center cells in the lymph node, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland and prostate, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, Leydig cells in the testis, glandular cells in the adrenal gland, breast and cervix and uterine and epididymis. We were unable to detect Alpha-1-Antichymotrypsin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Alpha-1-Antichymotrypsin expression as measured by anti-Alpha-1-Antichymotrypsin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - - - - - - - - - - - - - - - - - -
SERPINA3 Variability ++ ++ + + + + ++ ++ + + + + + ++ + + + + + +

Limitations and Warranty

enQuire Bio's Alpha-1-Antichymotrypsin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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