Immunohistochemical staining of alpha-1-Antitrypsin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

alpha-1-Antitrypsin [Polyclonal] Antibody (cGMP).

$ 128.00$ 208.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD213
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

Product NumberDescriptionPrice
QIVD213-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD213-0.5ml Size: 0.5 ml, Format: Concentrate $ 138.00
QIVD213-1ml Size: 1 ml, Format: Concentrate $ 208.00
QIVD213-6ml Size: 6 ml, Format: Predilute $ 128.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

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SKU: QIVD213-0.1ml
alpha-1-Antitrypsin General Information
Alternate Names
Molecular Weight
46.7 kDa
Chromosomal Location
[chr: CHR_HSCHR14_7_CTG1] [chr_start: 94378757] [chr_end: 94392703] [strand: -1]; q32.13 [chr: 14] [chr_start: 94376747] [chr_end: 94390693] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolSERPINA1
Entrez Gene ID5265
RefSeq Protein Accession(s)NP_001002236; NP_001121176; NP_001121179; XP_016876859; NP_001121178; NP_000286; NP_001121173; NP_001121172; NP_001121175; NP_001002235; NP_001121174; NP_001121177
RefSeq mRNA Accession(s)NM_000295; NM_001127707; NM_001127701; NM_001002235; NM_001002236; NM_001127702; NM_001127706; XM_017021370; NM_001127700; NM_001127703; NM_001127704; NM_001127705
RefSeq Genomic Accession(s)NC_000014; NG_008290; NT_187601
UniProt ID(s)P01009
PharmGKB ID(s)PA35509
KEGG Gene ID(s)hsa:5265
Associated Diseases (KEGG IDs)Alpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]: A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. {ECO:0000269|PubMed:1905728, ECO:0000269|PubMed:2227940, ECO:0000269|PubMed:2390072}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of alpha-1-Antitrypsin .
The antibody is specific against human alpha-1-antitrypsin. This antibody stains macrophages in human tissues and does not stain any other cell type.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-alpha-1-Antitrypsin Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: alpha-1-antitrypsin isolated from human serum.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

alpha-1-Antitrypsin Information for Pathologists

Summary:

Acute phase plasma protein predominantly derived from liver which inhibits neutrophil elastase. Most abundant circulating serine protease inhibitor. Elastase digests lung tissue and is secreted by neutrophils during inflammation (Wikipedia). Member of serpin superfamily; homologous to alpha-1-antichymotrypsin. Clinical features

Notable Clinical Features:

Common genetic deficiency, see Liver nontumor chapter. Occurs in 1 per 2,500-5,000 newborns in Western Europe / USA. Incidence is highly dependent on Scandinavian descent within the population. Disorder due to folding errors (IUBMB Life 2009;61:1). Causes emphysema (Orphanet J Rare Dis 2008;3:16), chronic hepatitis (Hum Pathol 2012;43:753) or cirrhosis (Am J Gastroenterol 2008;103:2136, Clin Gastroenterol Hepatol 2012;10:575).

Common Uses By Pathologists:

Immunohistochemistry marker of alpha-1-antitrypsin genetic disease in hepatocytes. Also marker of normal histiocytes and hepatocytes.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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alpha-1-Antitrypsin [Polyclonal] Antibody (cGMP).