Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:25-1:50|
|Pretreatment||No Pretreatment Required|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-alpha-1-Antitrypsin Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: alpha-1-antitrypsin isolated from human serum.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
alpha-1-Antitrypsin Information for Pathologists
Summary:Acute phase plasma protein predominantly derived from liver which inhibits neutrophil elastase. Most abundant circulating serine protease inhibitor. Elastase digests lung tissue and is secreted by neutrophils during inflammation (Wikipedia). Member of serpin superfamily; homologous to alpha-1-antichymotrypsin. Clinical features
Notable Clinical Features:Common genetic deficiency, see Liver nontumor chapter. Occurs in 1 per 2,500-5,000 newborns in Western Europe / USA. Incidence is highly dependent on Scandinavian descent within the population. Disorder due to folding errors (IUBMB Life 2009;61:1). Causes emphysema (Orphanet J Rare Dis 2008;3:16), chronic hepatitis (Hum Pathol 2012;43:753) or cirrhosis (Am J Gastroenterol 2008;103:2136, Clin Gastroenterol Hepatol 2012;10:575).
Common Uses By Pathologists:Immunohistochemistry marker of alpha-1-antitrypsin genetic disease in hepatocytes. Also marker of normal histiocytes and hepatocytes.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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