Immunohistochemical staining of alpha-1-Fetoprotein  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

alpha-1-Fetoprotein [Polyclonal] Antibody (cGMP).

$ 135.00$ 195.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

Product NumberDescriptionPrice
QIVD218-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD218-0.5ml Size: 0.5 ml, Format: Concentrate $ 155.00
QIVD218-1ml Size: 1 ml, Format: Concentrate $ 195.00
QIVD218-6ml Size: 6 ml, Format: Predilute $ 175.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD218-0.1ml
alpha-1-Fetoprotein General Information
Alternate Names
Molecular Weight
68.7 kDa
Chromosomal Location
q13.3 [chr: 4] [chr_start: 73431138] [chr_end: 73456174] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolAFP
Entrez Gene ID174
RefSeq Protein Accession(s)NP_001125
RefSeq mRNA Accession(s); NM_001134; NM_001354717
RefSeq Genomic Accession(s)NC_000004; NG_023028
UniProt ID(s)P02771
PharmGKB ID(s)PA24614
KEGG Gene ID(s)hsa:174
Associated Diseases (KEGG IDs)Alpha-fetoprotein deficiency (AFPD) [MIM:615969]: A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development. {ECO:0000269|PubMed:15280901, ECO:0000269|PubMed:18854864}. The disease is caused by mutations affecting the gene represented in this entry.; Alpha-fetoprotein, hereditary persistence (HPAFP) [MIM:615970]: A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life. {ECO:0000269|PubMed:7684942}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of alpha-1-Fetoprotein .
This antibody reacts with alpha-1-fetoprotein. A positive staining in the hepatocytes of fetal tissue was observed. This antibody does not react with any other cell or tissue types.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:150
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at RoomTemperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-alpha-1-Fetoprotein Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Fetal liver
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: alpha-1-fetoprotein isolated from human cord serum.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

alpha-1-Fetoprotein Information for Pathologists


Major plasma protein of early fetus; present in fetal gut, liver and yolk sac (Wikipedia). Serum levels may be elevated if severe hypothyroidism (J Pediatr Endocrinol Metab 2012;25:761). Uses by pathologists Serum marker:. Elevated in maternal serum or amniotic fluid ( > 2.5 multiples of median at a specified gestational age) if fetal developmental anomalies.

Common Uses By Pathologists:

Serum marker:. Elevated in maternal serum or amniotic fluid ( > 2.5 multiples of median at a specified gestational age) if fetal developmental anomalies. Serum tumor marker elevated in patients with hepatoblastoma (eMedicine), hepatocellular carcinoma, hepatoid adenocarcinoma, testicular cancer (other than pure seminoma) and yolk sac tumor, but not in intrahepatic cholangiocarcinoma (Am Surg 2010;76:1210). Preoperative levels are prognostic for hepatocellular carcinoma (World J Surg Oncol 2013;11:212). Immunostain:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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alpha-1-Fetoprotein [Polyclonal] Antibody (cGMP).