FFPE human prostate carcinoma sections stained with 100 ul anti-Androgen Receptor (clone SPM335) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.
FFPE human prostate carcinoma sections stained with 100 ul anti-Androgen Receptor (clone SPM335) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.

Anti-Androgen Receptor Antibody Clone SPM335

$ 298.00
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SKU: 367-MSM1X
Species: Human and Mouse (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
367-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 367-MSM1X-P1
Androgen Receptor General Information
Alternate Names
AR, Androgen Receptor, NR3C4
Molecular Weight
110kDa
Chromosomal Location
Xq12
Curated Database and Bioinformatic Data
Gene SymbolAR
Entrez Gene ID367
Ensemble Gene IDENSG00000169083
RefSeq Protein Accession(s)NP_000035, NP_001334992, NP_001334993, NP_001011645, NP_001334990
RefSeq mRNA Accession(s)NM_001348063, NM_001348064, NM_001348061, NM_000044
RefSeq Genomic Accession(s)NC_018934, NG_009014, NC_000023
UniProt ID(s)F1D8N5, A0A087WUX9, G4VV16, Q9NUA2, P10275
UniGene ID(s)F1D8N5, A0A087WUX9, G4VV16, Q9NUA2, P10275
HGNC ID(s)644
Cosmic ID(s)AR
KEGG Gene ID(s)hsa:367
PharmGKB ID(s)PA57
General Description of Androgen Receptor.
This antibody reacts with a 110kDa protein in western blot which is consistent with the known staining for androgen receptor (AR). This antibody reacts specifically with the newly described A form of the receptor. It does not cross react with the estrogen, progesterone, or glucocorticoid receptors. The androgen receptor plays a normal physiological role in a number of common effects associated with exposure to androgenic hormones. Under pathophysiological conditions, androgen receptor expression is often measured as a therapeutic marker in prostate cancer. Patients with tumors expressing high levels of androgen receptor generally benefit from hormonal therapy like androgen blockade. Additionally, androgen receptor protein levels in prostate carcinoma are sometimes labeled with an anti-AR antibody to estimate tumor differentiation and type. Generally, higher levels of AR expression are associated with lower tumor differentiation. Morpheaform basal cell carcinoma (mBCC) and desmoplastic trichoepithelioma (DTE), two common types of skin cancers exhibit differential expression of androgen receptor and can be differentiated by anti-androgen receptor antibody staining patterns. This monoclonal antibody is superb for staining of formalin/paraffin tissues.
What do we see in this antibody?Anti-androgen receptor antibody SPM335 was selected for its excellent specificity in immunohistochemistry as well as for its ability to stain cells for flow cytometry. Unlike clone, DHTR/882, clone SPM335 was generated using only amino acids 299-315 which are not in an active modulating or ligand binding site of human androgen receptor. In our IHC testing, anti-AR staining by clone SPM335 can be primarily observed in glandular epithelial and tumor cells in human prostate carcinoma tissue samples. This finding is consistent with the literature and supports the specificity of anti-AR antibody SPM335.

Human and Mouse (-) Anti-Androgen Receptor Antibody Product Attributes

Species: Human and Mouse (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Androgen Receptor Antibody Clone: SPM335
Clone SPM335 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse (-) Androgen Receptor Positive Control Sample: LNCap cells or Prostate carcinoma
Cellular Localization of Antibody SPM335 Staining: Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide, aa 299-315, (STEDTAEYSPFKGGYTK) of human AR
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Androgen Receptor Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ar antibody staining is expected to be primarily localized to the cytoplasm and nucleus.

Observed Antibody Staining Data By Tissue Type:

Variations in Androgen Receptor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the epididymis, seminal vesicle. More moderate antibody staining intensity was present in glandular cells in the epididymis, seminal vesicle. Low, but measureable presence of Androgen Receptor could be seen inglandular cells in the cervix, uterine. We were unable to detect Androgen Receptor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Androgen Receptor expression as measured by anti-Androgen Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - - - - - - - - - - - - +++ - - - - - -
AR Variability ++ + + + ++ + ++ + + + + ++ + + + ++ + + + +

Limitations and Warranty

enQuire Bio's Androgen Receptor Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Androgen Receptor Antibody Clone SPM335

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