Human PBMC were stained with PE Human Anti-CD4 , FITC Human Anti-CD45RO  followed by intracellular staining with 5 uL  (solid line) or 0.125 ug APC Mouse IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD152 / CTLA-4 Antibody [BNI3]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated ;APC
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB59-PE-25Tests
Anti-CD152 General Information
Alternate Names
Curated Database and Bioinformatic Data
Gene SymbolCTLA4
Entrez Gene ID1493
Ensemble Gene IDENSG00000163599
RefSeq Protein Accession(s)NP_005205, NP_001032720
RefSeq mRNA Accession(s)NM_001037631, NM_005214,
RefSeq Genomic Accession(s)NG_011502, NC_000002, NC_018913
UniProt ID(s)P16410
UniGene ID(s)P16410
HGNC ID(s)2505
Cosmic ID(s)CTLA4
KEGG Gene ID(s)hsa:1493
PharmGKB ID(s)PA27006
General Description of Anti-CD152.
The BNI3 antibody is specific for human CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions. The BNI3 antibody may be used for flow cytometric analysis of intracellular or surface CTLA-4 expression, and is also widely used for neutralization of CTLA-4 when expressed at the cell surface. The BNI3 antibody is reported to be cross-reactive with Baboon, Cynomolgus and Rhesus CTLA-4. Please choose the appropriate format for each application.

Human Anti-Anti-CD152 Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-Anti-CD152 Antibody Clone: BNI3
Clone BNI3 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD152 Previously Observed Antibody Staining Patterns

Selected References

Moreno-Fernandez ME, Rueda CM, Rusie LK, and Chougnet CA. 2011. Blood. 117: 5372-5380. (in vitro blocking)Schonfeld D, Matschiner G, Chatwell L, Trentmann S, Gille H, Hulsmeyer M, Brown N, Kaye PM, Schlehuber S, Hohlbaum AM and Skerra A. 2009. 106: 8198-8203. (Immunohistochemistry - frozen tissue)Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)Bonzheim I, Geissinger E, Tinguely M, Roth S, Grieb T, Reimer P, Wilhelm M, Rosenwald A, Muller-Hermelink HK, and Rudiger T. 2008. Am. J. Clin. Pathol. 130: 613-619. (Immunohistochemistry - paraffin embedded tissue; Immunofluorescence microscopy - frozen tissue)Young NT, Waller ECP, Patel R, Roghanian A, Austyn JM, and Trowsdale J. 2008. 111: 3090-3096. (in vitro activation)Wei B, da Rocha Dias S, Wang H and Rudd CE. 2007. J. Immunol. 179: 400-408. (in vitro activation)Jonuleit H, Schmitt E, Stassen M, Tuettenberg A, Knop J and Enk AH. 2001. J. Exp. Med. 193: 1285-1294 (in vitro blocking)Oaks MK and Hallett KM. 2000. J. Immunol. 164: 5015-5018. (Immunoprecipitation; EIA - plate coating)

Limitations and Warranty

enQuire Bio's Human Anti-CD152 / CTLA-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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