enQuire-Bio-QAB88-B-100ug-anti-Anti-CD154-TNFSF5-antibody-10
C57Bl/6 T cells, enriched from total splenocytes, were stimulated with PMA and ionomycin for 6 hours and stained with 0.25 ug FITC Mouse Anti-CD154 (QAB88) (solid line) or 0.25 ug FITC Armenian Hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 T cells, enriched from total splenocytes, were stimulated with PMA and ionomycin for 6 hours and stained with 0.06 ug PE Mouse Anti-CD154 (QAB88) (solid line) or 0.06 ug PE Armenian Hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD154 Antibody [MR1]

$ 48.00$ 138.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB88
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;FITC ;PE
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB88-B-25ug
Anti-CD154 / TNFSF5 General Information
Alternate Names
CD4L, CD154, TNFSF5, CD154, IGM, Ly-62, TRAP, Tnlg8b, HIGM1, CD4-L, Cd4l, Ly62, T-BAM, IMD3, Tnfsf5, gp39
Curated Database and Bioinformatic Data
Gene SymbolCd40lg
Entrez Gene ID21947
Ensemble Gene IDENSMUSG00000031132
RefSeq Protein Accession(s)NP_035746
RefSeq mRNA Accession(s)NM_011616
RefSeq Genomic Accession(s)NC_000086
UniProt ID(s)Q0VEI3, P27548
UniGene ID(s)Q0VEI3, P27548
Cosmic ID(s)Cd40lg
KEGG Gene ID(s)mmu:21947
General Description of Anti-CD154 / TNFSF5.
The MR1 antibody is specific for mouse CD154, a 39 kD glycoprotein that is primarily expressed on activated T cells. CD154 (CD40 ligand, gp39) binds to CD40 expressed on antigen presenting cells and mediates important costimulatory functions between these cells. Signals generated via cognate interactions between CD154-CD40 on activated T and B cells are required for T-dependent B cell responses. The MR1 antibody is reported to block interaction of CD154 with CD40.

Mouse Anti-Anti-CD154 / TNFSF5 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD154 / TNFSF5 Antibody Clone: MR1
Clone MR1 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD154 / TNFSF5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD40L / CD154 / TNFSF5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD40L / CD154 / TNFSF5 could be seen inhematopoietic cells in the bone marrow. We were unable to detect CD40L / CD154 / TNFSF5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. 1992. Proc Natl Acad Sci U S A. 89(14):6550-6554.Foy TM, Laman JD, Ledbetter JA, Aruffo A, Claassen E, Noelle RJ. 1994. J Exp Med. 180(1):157-163.Laman JD, Claassen E, Noelle RJ. 1996. Crit Rev Immunol. 16(1):59-108.Grewal IS, Flavell RA. 1998. Annu Rev Immunol. 16:111-35.

Limitations and Warranty

enQuire Bio's Mouse Anti-CD154 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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