Mouse Anti-Anti-CD273 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC), immunofluorescence, immunoprecipitation, Western Blot.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD273 Antibody Clone: TY25
Clone TY25 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CD273 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-PDCD1LG2 antibody staining is expected to be primarily localized to the cytosol.
Hams E, McCarron MJ, Amu S, Yagita H, Azuma M, Chen L, and Fallon PG. 2011. J. Immunol. 186:5648-5655. (in vivo blocking)Shen L, Lin Y, Freeman GJ, Sharpe AH, and Dana MR. 2007. J. Immunol. 179:3672-3679. (Immunohistochemistry - OCT embedded frozen tissue; Flow cytometry)Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, and Wuthrich RP. 2004. Nephrol. Dial. Transplant. 19: 2713-2720. (Immunofluorescence microscopy - frozen tissue)Ansari MJI, Salama AD, Chitnis T, Smith RN, Yagita H, Akiba H, Yamazaki T, Azuma M, Isai H, Khoury SJ, Auchincloss H, and Sayegh MH. 2003. J. Exp. Med. 198:63-71. (in vivo blocking, Immunohistochemistry - frozen tissue)Yamazaki T, Akiba H, Iwai H, Matsuda H, Aoki M, Tanno Y, Shin T, Tsuchiya H, Pardoll DM, Okumura K, Azuma M, and Yagita H. 2002. J. Immunol. 169: 5538-5545. (Immunoprecipitation, Western Blot)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD273/PD-L2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.