Mouse Anti-CD274 / PD-L1 Antibody [10F.9G2]

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SKU: QAB95
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB95-PE-25ug
Anti-CD274 General Information
Alternate Names
PD-L1, B7-H1, CD274, A5345L16Rik, Pdcd1l1, Pdl1, B7h1, Pdcd1lg1
Curated Database and Bioinformatic Data
Gene SymbolCd274
Entrez Gene ID60533
Ensemble Gene IDENSMUSG00000016496
RefSeq Protein Accession(s)NP_068693
RefSeq mRNA Accession(s)NM_021893
RefSeq Genomic Accession(s)NC_000085
UniProt ID(s)Q3U472, Q9EP73
UniGene ID(s)Q3U472, Q9EP73
Cosmic ID(s)Cd274
KEGG Gene ID(s)mmu:60533
General Description of Anti-CD274.
The 10F.9G2 antibody is specific for mouse CD274, more commonly known as PD-L1 or B7-H1, which acts as a ligand for the T cell co-regulatory receptor PD-1 (CD279). This interaction modulates T cell antigen receptor (TCR) signaling and therefore T cell activation. PD-L1 binding to PD-1 expressed on CD4- CD8- thymocytes participates in the processes of clonal selection, elimination of autoreactive lymphocytes, and development of tolerance. PD-L1 may also bind PD-1 following the receptors inducible expression on activated, mature T cells, where it has been proposed to limit T cell activation. PD-L1 is one of a group of B7 ligands whose interactions with the CD28 receptor family, also including CTLA-4 (CD152), provide a balance of co-stimulatory /co-inhibitory signaling important in T cell activation, tolerance, and autoimmunity.The 10F.9G2 antibody may be used as a marker for PD-L1 expression on T and B cells, NK cells and on dendritic cells. It is also widely used for analysis of receptor-ligand interaction and function(s) in vitro and in vivo.

Mouse Anti-Anti-CD274 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD274 Antibody Clone: 10F.9G2
Clone 10F.9G2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD274 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-PD-L1 / B7-H1 / CD274 antibody reveals PD-L1 / B7-H1 / CD274 expression is expected to be primarily localized to the cytosol.

Selected References

Chen A, Ahlen G, Brenndorfer ED, Brass A, Holmstrom F, Chen M, Soderholm J, Milich DR, Frelin L, and Sallberg M. 2011. J. Immunol. 186:5107-5118. (in vivo depletion)Zhang L, Gajewski TF, and Kline J. 2009. Blood. 114:1545-1552. (in vivo blocking)Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)Brooks DG, Ha S-J, Elsaesser H, Sharpe AH, Freeman GJ, and Oldstone MBA. 2008. Proc. Natl. Acad. Sci. 105:20428-20433. (in vivo blocking)Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD274 / PD-L1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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