C57Bl/6 splenocytes were stained with 0.25 ug APC Mouse Anti-CD3e (QAB2) (solid line) or 0.25 ug APC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.
C57Bl/6 splenocytes were stained with 0.25 ug APC Mouse Anti-CD3e (QAB2) (solid line) or 0.25 ug APC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 1 ug APC-Cy7 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 0.25 ug FITC Mouse Anti-CD3e  (solid line) or 0.25 ug FITC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 1 ug Mouse Anti-CD3e PE (QAB2) (solid line) or 1 ug Armenian hamster IgG PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug PE-Cy5 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 1 ug PE-Cy7 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 0.25 ug APC Mouse Anti-CD3e (QAB2) (solid line) or 0.25 ug APC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 1 ug APC-Cy7 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 0.25 ug FITC Mouse Anti-CD3e  (solid line) or 0.25 ug FITC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 1 ug Mouse Anti-CD3e PE (QAB2) (solid line) or 1 ug Armenian hamster IgG PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug PE-Cy5 Mouse Anti-CD3e .C57Bl/6 splenocytes were stained with 1 ug PE-Cy7 Mouse Anti-CD3e .

Anti-CD3e Antibody [145-2C11]

$ 48.00$ 318.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB2
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;APC-Cy7
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB2-B-25ug
Anti-CD3 epsilon General Information
Alternate Names
CD3e, CD3 epsilon, T3E, IMD18, TCRE
Curated Database and Bioinformatic Data
Gene SymbolCd3e
Entrez Gene ID12501
Ensemble Gene IDENSMUSG00000032093
RefSeq Protein Accession(s)NP_031674, XP_006510029
RefSeq mRNA Accession(s)XM_006509966, NM_007648
RefSeq Genomic Accession(s)NC_000075
UniProt ID(s)A6H6M1, P22646
UniGene ID(s)A6H6M1, P22646
Cosmic ID(s)Cd3e
KEGG Gene ID(s)mmu:12501
General Description of Anti-CD3 epsilon.
The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

Mouse Anti-Anti-CD3 epsilon Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD3 epsilon Antibody Clone: 145-2C11
Clone 145-2C11 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD3 epsilon Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3e / CD3 epsilon antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3e / CD3 epsilon could be seen in. We were unable to detect CD3e / CD3 epsilon in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Staehli R, Ludigs K, Heinz LX, Sequin-Estevez Q, Ferero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. (in vitro activation)Todo T, Wu G, Chai NN, He Y, Martins G, Gupta A, Fair J, Liu NY, Jordan S, and Klein A. 2012. Int. Immunol. 10:1093. (in vivo assay)Mira E, Leon B, Barber DF, Jimenez-Baranda S, Goya I, Almonacid L, Marquez G, Zaballos A, Martinez C, Stein JV, Ardavin C and Manes S. 2012. J. Immunol. (in vitro activation, Immunohistochemistry - frozen tissue)Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. (Immunofluorescence microscopy - OCT embedded frozen tissue)Salmond RJ, Filby A, Pirinen N, Magee AI, and Zamoyska R. 2010. Blood. 117: 108-117. (Immunoprecipitation)Tilley SL, Jaradat M, Stapleton C, Dixon D, Hua X, Erikson CJ,. McCaskill JG, Chason KD, Liao G, Jania L, Koller BH, and Jetten AM. 2007. J. Immunol. 178: 3208-18. (Immunohistochemistry - frozen tissue)Isakov N, Wange RL, Burgess WH, Watts JD, Aebersold R, and Samelson LE. 1995. J. Exp. Med. 181:375-380. (in vitro activation, Immunoprecipitation)Salvadori S, Gansbacher B, Pizzimenti AM, and Zier KS. 1994. J. Immunol. 153: 5176 - 5182. (Western Blot)Leo O, Foo M, Sachs D, Samuelson L, and Bluestone J. 1987. Proc. Natl. Acad. Sci. USA. 84: 1374 (Origination of clone 145-2C11, in vitro activation, in vitro blocking, Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD3e Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD3e Antibody [145-2C11]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

Anti-CD3e Antibody [145-2C11]https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-APC-100ug-anti-Anti-CD3-epsilon-antibody-10-292x300.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-APC7-100ug-anti-Anti-CD3-epsilon-antibody-10-300x277.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-B-100ug-anti-Anti-CD3-epsilon-antibody-10-300x293.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-F-100ug-anti-Anti-CD3-epsilon-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-PCP55-100ug-anti-Anti-CD3-epsilon-antibody-10-300x294.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-PE-100ug-anti-Anti-CD3-epsilon-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-PE5-100ug-anti-Anti-CD3-epsilon-antibody-10-300x300.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB2-PE7-100ug-anti-Anti-CD3-epsilon-antibody-10-292x300.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-APC-100ug-anti-Anti-CD3-epsilon-antibody-9-292x300.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-APC7-100ug-anti-Anti-CD3-epsilon-antibody-9-300x277.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-F-100ug-anti-Anti-CD3-epsilon-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-PCP55-100ug-anti-Anti-CD3-epsilon-antibody-9-300x294.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-PE-100ug-anti-Anti-CD3-epsilon-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-PE5-100ug-anti-Anti-CD3-epsilon-antibody-9-300x300.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB2-PE7-100ug-anti-Anti-CD3-epsilon-antibody-9-292x300.png