C57Bl/6 splenocytes were stained with 0.25 ug APC Mouse Anti-CD3e (QAB2) (solid line) or 0.25 ug APC Armenian hamster IgG isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD3e Antibody [145-2C11]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;APC-Cy7
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB2-B-25ug
Anti-CD3 epsilon General Information
Alternate Names
CD3e, CD3 epsilon, T3E, IMD18, TCRE
Curated Database and Bioinformatic Data
Gene SymbolCd3e
Entrez Gene ID12501
Ensemble Gene IDENSMUSG00000032093
RefSeq Protein Accession(s)NP_031674, XP_006510029
RefSeq mRNA Accession(s)XM_006509966, NM_007648
RefSeq Genomic Accession(s)NC_000075
UniProt ID(s)A6H6M1, P22646
UniGene ID(s)A6H6M1, P22646
Cosmic ID(s)Cd3e
KEGG Gene ID(s)mmu:12501
General Description of Anti-CD3 epsilon.
The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

Mouse Anti-Anti-CD3 epsilon Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD3 epsilon Antibody Clone: 145-2C11
Clone 145-2C11 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD3 epsilon Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3e / CD3 epsilon antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3e / CD3 epsilon could be seen in. We were unable to detect CD3e / CD3 epsilon in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Staehli R, Ludigs K, Heinz LX, Sequin-Estevez Q, Ferero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. (in vitro activation)Todo T, Wu G, Chai NN, He Y, Martins G, Gupta A, Fair J, Liu NY, Jordan S, and Klein A. 2012. Int. Immunol. 10:1093. (in vivo assay)Mira E, Leon B, Barber DF, Jimenez-Baranda S, Goya I, Almonacid L, Marquez G, Zaballos A, Martinez C, Stein JV, Ardavin C and Manes S. 2012. J. Immunol. (in vitro activation, Immunohistochemistry - frozen tissue)Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. (Immunofluorescence microscopy - OCT embedded frozen tissue)Salmond RJ, Filby A, Pirinen N, Magee AI, and Zamoyska R. 2010. Blood. 117: 108-117. (Immunoprecipitation)Tilley SL, Jaradat M, Stapleton C, Dixon D, Hua X, Erikson CJ,. McCaskill JG, Chason KD, Liao G, Jania L, Koller BH, and Jetten AM. 2007. J. Immunol. 178: 3208-18. (Immunohistochemistry - frozen tissue)Isakov N, Wange RL, Burgess WH, Watts JD, Aebersold R, and Samelson LE. 1995. J. Exp. Med. 181:375-380. (in vitro activation, Immunoprecipitation)Salvadori S, Gansbacher B, Pizzimenti AM, and Zier KS. 1994. J. Immunol. 153: 5176 - 5182. (Western Blot)Leo O, Foo M, Sachs D, Samuelson L, and Bluestone J. 1987. Proc. Natl. Acad. Sci. USA. 84: 1374 (Origination of clone 145-2C11, in vitro activation, in vitro blocking, Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD3e Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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