C57Bl/6 splenocytes were stained with 0.25 ug Biotin Mouse Anti-CD16/CD32 (QAB87) (solid line) or 0.25 ug Biotin Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events., followed by Streptavidin PE.

Mouse Anti-CD32 Antibody [2.4G2]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;Biotin ;PE ;PE-Cy7
Isotype: Rat IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB87-F-25ug
Anti-CD32 General Information
Alternate Names
FCGR2b, CD32b, Low affinity immunoglobulin gamma Fc region receptor IIb
Curated Database and Bioinformatic Data
Gene SymbolFCGR2b
Entrez Gene ID14131
Ensemble Gene IDENSMUSG00000059498
RefSeq Protein Accession(s)NP_034318, XP_006496721, XP_006496724, XP_006496723, XP_006496722
RefSeq mRNA Accession(s)NM_010188, XM_006496660, XM_006496661, XM_006496658, XM_006496659
RefSeq Genomic Accession(s)NC_000067
UniProt ID(s)E9PUV2, A0A0B4J1M6
UniGene ID(s)E9PUV2, A0A0B4J1M6
Cosmic ID(s)Fcgr3
KEGG Gene ID(s)mmu:14131
General Description of Anti-CD32.
The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). These receptors are expressed by B cells, monocytes, macrophages, NK cells, dendritic cells, and neutrophils.

Mouse Anti-Anti-CD32 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Anti-CD32 Antibody Clone: 2.4G2
Clone 2.4G2 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CD32 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD16 / CD32 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD16 / CD32 could be seen in cells in the seminiferous ducts in testis, epidermal cells in the skin, exocrine glandular cells in the pancreas, germinal center cells in the lymph node, glandular cells in the epididymis, fallopian tube, gallbladder, salivary gland, stomach and thyroid gland, lymphoid tissue in appendix, macrophages in lung, myocytes in heart muscle, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix and uterine, oral mucosa and vagina. We were unable to detect CD16 / CD32 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Blocking- Immunofluorescence microscopy)Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge et al. 2012. J. Immunol. 188:2524-2536. (Blocking - in vitro)Shimazu T, Iida R, Zhang Q, Welner RS, Medina KL, Alberola-Ila J, and Kincade PW. 2012. Blood. 119:4889-4897. (Blocking - Flow cytometry)Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, LaVoy A, Miller M, Klaenhammer TR, and Dean GA. 2011. Clin. Vaccine Immunol. 18: 1834-1844. (Blocking - Flow cytometry)Beretta F, St.-Pierre J, Piccirillo CA, and Stevenson MM. 2011. J. Immunol. 186: 4862-4871. (Blocking - Flow cytometry)Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD32 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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