Human Anti-Anti-CDAnti-CD95L Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-Anti-CDAnti-CD95L Antibody Clone: NOK-1
Clone NOK-1 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CDAnti-CD95L Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in Fas Ligand / CD178 / CD95L antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Fas Ligand / CD178 / CD95L could be seen in cells in the seminiferous ducts in testis, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, duodenum, epididymis, fallopian tube, small intestine, stomach and thyroid gland, islets of Langerhans in pancreas, lymphoid tissue in appendix, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, non-germinal center cells in the lymph node and tonsil, Purkinje cells in the cerebellum and squamous epithelial cells in the tonsil. We were unable to detect Fas Ligand / CD178 / CD95L in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of FasL / CD178 / CD95L expression as measured by anti-FasL / CD178 / CD95L antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Tanaka M, Suda T, Takahashi T, and Nagata S. 1995. EMBO J. 14(6):1129-1135. Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, and Yagita H. 1995. J Exp Med. 182(6):1777-1783. Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S. 1997. J Exp Med. 186(12):2045-2050. Ehrenschwender M, Wajant H. 2009. Adv Exp Med Biol. 647:64-93.
Limitations and Warranty
enQuire Bio's Human Anti-CD178 / Fas ligand Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.