Human CD178  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD178 / Fas ligand Antibody [NOK-1]

$ 89.00$ 219.00
Please Select A Product To View The SKU.

SKU: QAB85
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;PE
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB85-B-25ug
Anti-CDAnti-CD95L General Information
Alternate Names
Fas Ligand, FASLG, CD95L, TNFSF6, APT1LG1, TNLG1A, CD95-L, ALPS1B, FASL, CD95L, APTL, CD178
Curated Database and Bioinformatic Data
Gene SymbolFASLG
Entrez Gene ID356
Ensemble Gene IDENSG00000117560
RefSeq Protein Accession(s)NP_000630, NP_001289675
RefSeq mRNA Accession(s)NM_001302746, NM_000639
RefSeq Genomic Accession(s)NC_018912, NG_007269, NC_000001
UniProt ID(s)P48023, Q53ZZ1
UniGene ID(s)P48023, Q53ZZ1
HGNC ID(s)11936
Cosmic ID(s)FASLG
KEGG Gene ID(s)hsa:356
PharmGKB ID(s)PA56
General Description of Anti-CDAnti-CD95L.
The NOK-1 antibody reacts with human CD178 (Fas ligand) in both membrane bound and soluble forms. Fas ligand is a 40 kDa transmembrane glycoprotein, a member of the TNF family, and is expressed by activated T and NK cells, neutrophils, and monocytes. Interactions between CD178 (Fas ligand) and CD95 (Fas) induce a program of apoptosis and play a key role in immune regulation and homeostasis. The extracellular domain of human CD178 can be cleaved from the surface by matrix metalloproteinases (MMPs) resulting in a 26 kDa soluble protein.

Human Anti-Anti-CDAnti-CD95L Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-Anti-CDAnti-CD95L Antibody Clone: NOK-1
Clone NOK-1 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Anti-CDAnti-CD95L Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Fas Ligand / CD178 / CD95L antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Fas Ligand / CD178 / CD95L could be seen in cells in the seminiferous ducts in testis, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, duodenum, epididymis, fallopian tube, small intestine, stomach and thyroid gland, islets of Langerhans in pancreas, lymphoid tissue in appendix, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, non-germinal center cells in the lymph node and tonsil, Purkinje cells in the cerebellum and squamous epithelial cells in the tonsil. We were unable to detect Fas Ligand / CD178 / CD95L in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of FasL / CD178 / CD95L expression as measured by anti-FasL / CD178 / CD95L antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - + - - - - - - - - - - - - - + -
FASLG Variability + ++ + + ++ + ++ ++ + + + + ++ + + + + ++ ++ +

Selected References

Tanaka M, Suda T, Takahashi T, and Nagata S. 1995. EMBO J. 14(6):1129-1135. Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, and Yagita H. 1995. J Exp Med. 182(6):1777-1783. Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S. 1997. J Exp Med. 186(12):2045-2050. Ehrenschwender M, Wajant H. 2009. Adv Exp Med Biol. 647:64-93.

Limitations and Warranty

enQuire Bio's Human Anti-CD178 / Fas ligand Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Human Anti-CD178 / Fas ligand Antibody [NOK-1]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top