Data from SDS-PAGE analysis of Anti-Arginase 1 antibody (Clone ARG1/1125). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Western Blot Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).

Anti-Arginase 1 Antibody Clone ARG1/1125

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 383-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG3 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
383-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 383-MSM1-P1
Arginase 1 General Information
Alternate Names
Arginase, Arg1
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolARG1
Entrez Gene ID383
Ensemble Gene IDENSG00000118520
RefSeq Protein Accession(s)NP_001231367, NP_000036, XP_011534103
RefSeq mRNA Accession(s)NM_000045, NM_001244438
RefSeq Genomic Accession(s)NG_007086, NC_018917, NG_031860, NC_000006
UniProt ID(s)P05089
UniGene ID(s)P05089
HGNC ID(s)663
Cosmic ID(s)ARG1
KEGG Gene ID(s)hsa:383
PharmGKB ID(s)PA24947
General Description of Arginase 1.
Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine, urea. Arginase I, II are isoenzymes which differ in subcellular localization, regulation,, possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues, granulocytes in peripheral blood. ARG-1 is a sensitive, specific marker for identification of hepatocellular carcinoma.

Human Anti-Arginase 1 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Arginase 1 Antibody Clone: ARG1/1125
Clone ARG1/1125 Host and Isotype: Mouse IgG3 kappa
Anti-Human Arginase 1 Positive Control Sample: 293T cells. Hepatocellular Carcinoma (HCC).
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Arginase 1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Arginase 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hepatocytes in liver. More moderate antibody staining intensity was present in hepatocytes in liver. Low, but measureable presence of Arginase 1 could be seen inhematopoietic cells in the bone marrow. We were unable to detect Arginase 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Arginase 1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Arginase 1 Antibody Clone ARG1/1125 1 Specificity Assay (Array)-300x121.jpg 1 IHC human Hepatocellular Carcinoma (ARG1 1125)-300x225.jpg 1 (ARG1 1125) Gel-244x300.jpg WB recombinant protein and hu Liver (ARG 1125)-150x300.jpg