Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).
Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).

Anti-Aurora B Antibody Clone AURKB/1521

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 9212-MSM1
Species: Human
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
9212-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
9212-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
9212-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 518.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 9212-MSM1-P0
Aurora B General Information
Alternate Names
Aurora B kinase, AURKB
Molecular Weight
39kDa
Chromosomal Location
17p13.1
Curated Database and Bioinformatic Data
Gene SymbolAURKB
Entrez Gene ID9212
Ensemble Gene IDENSG00000178999
RefSeq Protein Accession(s)XP_016880797, XP_016880800, XP_016880798, NP_001271455, NP_001300879, NP_001300880, XP_011522372, NP_001243763, NP_001300883, NP_004208, XP_016880796, NP_001300881, NP_001300882, XP_011522374, XP_016880799, NP_001300884
RefSeq mRNA Accession(s)XM_011524070, XM_017025310, XM_017025311, NM_001313955, XM_017025309, NM_001256834, NM_001313951, NM_001313953, NM_001313952, NM_001313954, XM_017025307, NR_132730, NM_001284526, NM_001313950, NM_004217, NR_132731, XM_011524072, XM_017025308
RefSeq Genomic Accession(s)NC_000017, NC_018928
UniProt ID(s)Q96GD4
UniGene ID(s)Q96GD4
HGNC ID(s)11390
Cosmic ID(s)AURKB
KEGG Gene ID(s)hsa:9212
PharmGKB ID(s)PA36199
General Description of Aurora B.
Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Human Anti-Aurora B Antibody Product Attributes

Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-Aurora B Antibody Clone: AURKB/1521
Clone AURKB/1521 Host and Isotype: Mouse IgG2b
Anti-Human Aurora B Positive Control Sample: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma
Cellular Localization of Antibody Nuclear and Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Aurora B protein fragment (aa89-251)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Aurora B Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-AURKB antibody staining is expected to be primarily localized to the midbody and nucleoplasm. There is variability in either the signal strength or the localization of signal in midbody and nucleoplasm from cell to cell. Additionally, antibody labeling in the midbody is dependent upon phase within the cell cycle.

Observed Antibody Staining Data By Tissue Type:

Variations in Aurora B antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Aurora B could be seen in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, decidual cells in the placenta, epidermal cells in the skin, glandular cells in the adrenal gland, breast, colon, duodenum, endometrium, gallbladder, small intestine and stomach, glial cells in the cerebral cortex, keratinocytes in skin, macrophages in lung, myocytes in skeletal muscle, neuronal cells in the cerebral cortex, respiratory epithelial cells in the bronchus, smooth muscle cells in the smooth muscle, squamous epithelial cells in the esophagus and oral mucosa, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. We were unable to detect Aurora B in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Aurora B expression as measured by anti-Aurora B antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + ++ ++ + + ++ + + ++ ++ ++ + - + ++ ++ ++ + ++
AURKB Variability ++ ++ ++ ++ +++ ++ + ++ ++ ++ +++ ++ +++ ++ ++ ++ ++ ++ + ++

Limitations and Warranty

enQuire Bio's Aurora B Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Aurora B Antibody Clone AURKB/1521

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