FFPE human Hodgkin's Lymphoma labeled with Anti-human Bax Monoclonal Antibody (Clone SPM336). Antigen retrieval in TE buffer (pH 9.0).
FFPE human Hodgkin's Lymphoma labeled with Anti-human Bax Monoclonal Antibody (Clone SPM336). Antigen retrieval in TE buffer (pH 9.0).

Anti-Bax Antibody Clone SPM336

$ 0.00$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 581-MSM1X
Species: Human, Monkey, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
581-MSM1X-P1 Size: 20 ug, Tag: Unconjugated $ 0.00
581-MSM1X-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 581-MSM1X-P1
Bax General Information
Alternate Names
Apoptosis regulator BAX, BCL-2-like protein 4, Bcl-2-associated X protein
Molecular Weight
21kDa
Chromosomal Location
19q13.33
Curated Database and Bioinformatic Data
Gene Symbol BAX
Entrez Gene ID 581
Ensemble Gene ID ENSG00000087088
RefSeq Protein Accession(s) XP_016882566, NP_001278358, NP_620119, NP_004315, NP_001278360, NP_620118, NP_001278357, NP_620116, NP_001278359
RefSeq mRNA Accession(s) XM_017027077 NM_001291430, NM_004324, NM_001291431, NM_138763, NM_001291429, NM_001291428, NM_138764, NR_027882, NM_138761, NM_138762
RefSeq Genomic Accession(s) NC_018930, NC_000019, NG_012191
UniProt ID(s) Q5ZPJ1, I6LPK7, Q07812, Q5ZPJ0
UniGene ID(s) Q5ZPJ1, I6LPK7, Q07812, Q5ZPJ0
HGNC ID(s) 959
Cosmic ID(s) BAX
KEGG Gene ID(s) hsa:581
PharmGKB ID(s) PA25269
General Description of Bax.
Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax, shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2, it homodimerizes, forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line,, Bax also counters the death repressor activity of Bcl-2.

Human, Monkey, Mouse (-), and Rat (-) Anti-Bax Antibody Product Attributes

Species: Human, Monkey, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Bax Antibody Clone: SPM336
Clone SPM336 Host and Isotype: Mouse IgG1
Anti-Human, Monkey, Mouse (-), and Rat (-) Bax Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Bax Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Bax antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, gallbladder, parathyroid gland, prostate, rectum, small intestine, stomach and thyroid gland, Leydig cells in the testis, macrophages in lung, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, gallbladder, parathyroid gland, prostate, rectum, small intestine, stomach and thyroid gland, Leydig cells in the testis, macrophages in lung, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Bax could be seen inadipocytes in breast and mesenchymal tissue, cells in the endometrial stroma in endometrium, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, follicle cells in the ovary, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hepatocytes in liver, Langerhans in skin, lymphoid tissue in appendix, myocytes in skeletal muscle, neuropil in cerebral cortex, non-germinal center cells in the tonsil, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue, peripheral nerve/ganglion in colon, pneumocytes in lung, smooth muscle cells in the smooth muscle and squamous epithelial cells in the vagina. We were unable to detect Bax in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bax expression as measured by anti-Bax antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ +++ ++ ++ ++ ++ ++ ++ ++ ++ ++
BAX Variability ++ ++ ++ + ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + ++ ++ ++ +

Limitations and Warranty

enQuire Bio's Bax Anti-Human, Monkey Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Bax Antibody Clone SPM336

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