Staining with Mouse Monoclonal BCL-2 [100 D5 + 124] Antibody in formalin-fixed paraffin-embedded human non-Hodgkins Lymphoma. Note cytoplasmic and nuclear membrane staining.
Staining with Mouse Monoclonal BCL-2 [100 D5 + 124] Antibody in formalin-fixed paraffin-embedded human non-Hodgkins Lymphoma. Note cytoplasmic and nuclear membrane staining.Staining with mouse monoclonal Bcl-2 [Clone 100/D5 + 124] antibody in Jurkat cells. See intercellular staining (red) and isotype control (green).Western Blot: Human skin lysate, 10ug. Using Mouse monoclonal Bcl-2 [Clone 100/D5 + 124] antibody at 1:500.

Anti-Bcl-2 Antibody Clone 100/D5 + 124

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 596-MSM4
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
596-MSM4-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 596-MSM4-P1
Bcl-2 General Information
Alternate Names
Bcl-2, B-cell lymphoma 2, BCL2
Molecular Weight
25-26kDa
Chromosomal Location
18q21.33
Curated Database and Bioinformatic Data
Gene Symbol BCL2
Entrez Gene ID 596
Ensemble Gene ID ENSG00000171791
RefSeq Protein Accession(s) NP_000624, XP_016881406, NP_000648, XP_011524437
RefSeq mRNA Accession(s) XM_011526135 NM_000633, XR_935248, XM_017025917, NM_000657
RefSeq Genomic Accession(s) NG_009361, NC_000018, NC_018929
UniProt ID(s) A0A024R2B3, A0A024R2C4, P10415
UniGene ID(s) A0A024R2B3, A0A024R2C4, P10415
HGNC ID(s) 990
Cosmic ID(s) BCL2
KEGG Gene ID(s) hsa:596
PharmGKB ID(s) PA25302
General Description of Bcl-2.
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive, neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein, the small number in which the neoplastic cells are bcl-2 negative.

Human, Mouse (-), and Rat (-) Anti-Bcl-2 Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Bcl-2 Antibody Clone: 100/D5 + 124
Clone 100/D5 + 124 Host and Isotype: Mouse IgG
Anti-Human, Mouse (-), and Rat (-) Bcl-2 Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
Cellular Localization of Antibody <100/D5 + 124 Staining: Outer mitochondrial membranes, endoplasmic reticulum as well as nuclear membranes.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 (100/D5 & 124)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Bcl-2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-BCL2 antibody staining is expected to be primarily localized to the nuclear membrane and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Bcl-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Bcl-2 could be seen inadipocytes in breast and mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the duodenum, gallbladder and small intestine and squamous epithelial cells in the esophagus. We were unable to detect Bcl-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-2 expression as measured by anti-Bcl-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - - - + - - - - +++ +++ - - - ++ - - - - -
BCL2 Variability ++ ++ + ++ ++ ++ + + ++ ++ ++ ++ ++ + ++ ++ + + ++ +

Limitations and Warranty

enQuire Bio's Bcl-2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Bcl-2 Antibody Clone 100/D5 + 124

https://enquirebio.com/wp-content/uploads/2019/01/Bcl-2 IHC human non-Hodgkins Lymphoma (100 D5 + 124)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Bcl-2 Flow Cytometry Jurkat Cells (100 D5 + 124)-300x254.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Western Blot Bcl-2 human Skin lysate (100 D5 + 124)-300x293.jpg