Human Anti-Bcl-6 Antibody Product Attributes
Tested Applications: Immunohistochemistry (IHC).
Anti-Bcl-6 Antibody Clone: BCL6/1526
Clone BCL6/1526 Host and Isotype: Mouse IgG1
Anti-Human Bcl-6 Positive Control Sample: Raji or Ramos cells. Tonsil or Hodgkin s lymphoma.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human bcl-6 protein fragment (aa256-389)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Bcl-6 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-BCL6 antibody staining is expected to be primarily localized to the nucleoplasm.
Observed Antibody Staining Data By Tissue Type:Variations in Bcl-6 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow and respiratory epithelial cells in the bronchus and nasopharynx. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow and respiratory epithelial cells in the bronchus and nasopharynx. Low, but measureable presence of Bcl-6 could be seen in cells in the red pulp in spleen, epidermal cells in the skin, fibroblasts in mesenchymal tissue, glandular cells in the appendix, epididymis, rectum and stomach, keratinocytes in skin, Leydig cells in the testis, myocytes in skeletal muscle, neuronal cells in the cerebral cortex, non-germinal center cells in the lymph node and tonsil, peripheral nerve in mesenchymal tissue, Purkinje cells in the cerebellum, squamous epithelial cells in the oral mucosa and trophoblastic cells in the placenta. We were unable to detect Bcl-6 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-6 expression as measured by anti-Bcl-6 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Bcl-6 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.