Formalin-fixed, paraffin-embedded human Tonsil stained with bcl-6 Monoclonal Antibody (BCL6/1527).
Formalin-fixed, paraffin-embedded human Tonsil stained with bcl-6 Monoclonal Antibody (BCL6/1527).

Anti-Bcl-6 Antibody Clone BCL6/1527

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 604-MSM7
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
604-MSM7-P0 Size: 20 ug, Tag: Unconjugated $ 118.00
604-MSM7-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
604-MSM7-P2 Size: 200 ug, Tag: Unconjugated $ 418.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 604-MSM7-P0
Bcl-6 General Information
Alternate Names
B-cell lymphoma 6 protein, BCL6
Molecular Weight
95kDa
Chromosomal Location
3q27.3
Curated Database and Bioinformatic Data
Gene SymbolBCL6
Entrez Gene ID604
Ensemble Gene IDENSG00000113916
RefSeq Protein Accession(s)NP_001697, XP_005247751, NP_001128210, XP_011511364, NP_001124317
RefSeq mRNA Accession(s)NM_001130845 NM_001134738, NM_001706, NM_138931, XM_005247694, XM_011513062
RefSeq Genomic Accession(s)NC_018914, NC_000003, NG_007149
UniProt ID(s)B5B0A5, P41182
UniGene ID(s)B5B0A5, P41182
HGNC ID(s)1001
Cosmic ID(s)BCL6
KEGG Gene ID(s)hsa:604
PharmGKB ID(s)PA25312
General Description of Bcl-6.
Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Human Anti-Bcl-6 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Bcl-6 Antibody Clone: BCL6/1527
Clone BCL6/1527 Host and Isotype: Mouse IgG1 kappa
Anti-Human Bcl-6 Positive Control Sample: Raji or Ramos cells. Tonsil or Hodgkin s lymphoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human bcl-6 protein fragment (aa256-389)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Bcl-6 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-BCL6 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Bcl-6 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow and respiratory epithelial cells in the bronchus and nasopharynx. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow and respiratory epithelial cells in the bronchus and nasopharynx. Low, but measureable presence of Bcl-6 could be seen in cells in the red pulp in spleen, epidermal cells in the skin, fibroblasts in mesenchymal tissue, glandular cells in the appendix, epididymis, rectum and stomach, keratinocytes in skin, Leydig cells in the testis, myocytes in skeletal muscle, neuronal cells in the cerebral cortex, non-germinal center cells in the lymph node and tonsil, peripheral nerve in mesenchymal tissue, Purkinje cells in the cerebellum, squamous epithelial cells in the oral mucosa and trophoblastic cells in the placenta. We were unable to detect Bcl-6 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-6 expression as measured by anti-Bcl-6 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - + - - -
BCL6 Variability + + + + + + ++ + + ++ + + + + + + +++ + + +

Limitations and Warranty

enQuire Bio's Bcl-6 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Bcl-6 Antibody Clone BCL6/1527

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