Immunohistochemical staining of Bcl-6 Oncoprotein  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Bcl-6 Oncoprotein [LN22] Antibody (cGMP).

$ 128.00$ 638.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD71
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2b

Product NumberDescriptionPrice
QIVD71-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD71-0.5ml Size: 0.5 ml, Format: Concentrate $ 358.00
QIVD71-1ml Size: 1 ml, Format: Concentrate $ 638.00
QIVD71-6ml Size: 6 ml, Format: Predilute $ 218.00
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SKU: QIVD71-0.1ml
Bcl-6 Oncoprotein General Information
Alternate Names
Molecular Weight
78.8 kDa
Chromosomal Location
q27.3 [chr: 3] [chr_start: 187721377] [chr_end: 187745725] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolBCL6
Entrez Gene ID604
RefSeq Protein Accession(s)NP_001124317; NP_001128210; XP_011511364; XP_005247751; NP_001697
RefSeq mRNA Accession(s)NM_001130845; NM_001134738; NM_001706; NM_138931; XM_005247694; XM_011513062
RefSeq Genomic Accession(s)NC_000003; NG_007149
UniProt ID(s)P41182
PharmGKB ID(s)PA25312
KEGG Gene ID(s)hsa:604
Associated Diseases (KEGG IDs)Chromosomal aberrations involving BCL6 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL), including diffuse large B-cell lymphoma and follicular lymphoma. Approximately 40% of diffuse large B-cell lymphomas and 5 to 10% of follicular lymphomas are associated with chromosomal translocations that deregulate expression of BCL6 by juxtaposing heterologous promoters to the BCL6 coding domain (PubMed:10469447, PubMed:10753856, PubMed:12414651, PubMed:11821949). Translocation t(3;14)(q27;q32). Translocation t(3;22)(q27;q11) with immunoglobulin gene regions (PubMed:11821949). Translocation t(3;7)(q27;p12) with IKZF1 gene 5'non-coding region (PubMed:10753856). Translocation t(3;6)(q27;p21) with Histone H4 (PubMed:12414651). Translocation t(3;16)(q27;p11) with IL21R. Translocation t(3;13)(q27;q14) with LCP1 (PubMed:10469447). {ECO:0000269|PubMed:10469447, ECO:0000269|PubMed:10753856, ECO:0000269|PubMed:11821949, ECO:0000269|PubMed:12414651}.; A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.; A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
General Description of Bcl-6 Oncoprotein .
This antibody reacts with the Bcl-6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas, and in nodular, lymphocyte predominant Hodgkin’s disease. Bcl-6 is a proto-oncogene that encodes Kruppel-type Zinc-finger protein of 95 kD and shares homology with transcription factor. Bcl-6 is mainly expressed in normal germinal center B cells and related lymphomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Bcl-6 Oncoprotein Antibody Clone: LN22
Host and Isotype: Mouse IgG2b
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody LN22 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Prokaryotic recombinant protein corresponding to 1–350 amino acids of the N-terminus of human Bcl-6.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Bcl-6 Oncoprotein Information for Pathologists

Summary:

Transcription factor at 3q27 normally expressed in germinal centers and in 5 - 15% of intrafollicular CD4+ T cells. Protects germinal center (GC) B cells against DNA damage induced apoptosis during somatic hypermutation and class switch recombination (J Exp Med 2010;207:1209). Translocations help define double hit lymphomas (Am J Clin Pathol 2010;134:258). Interpretation Nuclear staining.

Common Uses By Pathologists:

Germinal center marker (also CD10). Marker for follicular lymphoma (30 - 80%) of cases, can distinguish from MALT (BCL6-). Double staining of CD3 and BCL6 is helpful in appreciating follicular T helper cell immunophenotype of angioimmunoblastic T cell lymphoma (Hum Pathol 2010;41:79). Microscopic (histologic) images Images hosted on other servers:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Bcl-6 Oncoprotein [LN22] Antibody (cGMP).