Western Blot of Jurkat Cell Lysate using Bcl-x Monoclonal Antibody (2H12).
Western Blot of Jurkat Cell Lysate using Bcl-x Monoclonal Antibody (2H12).

Anti-Bcl-X Antibody Clone 2H12

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 598-MSM2
Species: Human, Mouse, Rat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
598-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 598-MSM2-P1
Bcl-X General Information
Alternate Names
Bcl-2-like 1, BCL2L1
Molecular Weight
27kDa
Chromosomal Location
20q11.21
Curated Database and Bioinformatic Data
Gene SymbolBCL2L1
Entrez Gene ID598
Ensemble Gene IDENSG00000171552
RefSeq Protein Accession(s)NP_001182, NP_001304848, NP_001304849, NP_001304850, NP_001309168, NP_001309171, XP_011527266, XP_016883482, NP_001309169, NP_612815
RefSeq mRNA Accession(s)NM_001191, NM_001317920, NM_001322240, XM_011528964, XR_001754364, NM_001317921, NM_001322239, NM_001317919, NM_138578, NM_001322242, XM_017027993, XR_936599 NR_134257
RefSeq Genomic Accession(s)NC_000020, NG_029002, NC_018931
UniProt ID(s)Q5TE63, Q07817, A0A0S2Z3C5
UniGene ID(s)Q5TE63, Q07817, A0A0S2Z3C5
HGNC ID(s)992
Cosmic ID(s)BCL2L1
KEGG Gene ID(s)hsa:598
PharmGKB ID(s)PA76
General Description of Bcl-X.
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death., Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS, Bcl-XL proteins.

Human, Mouse, Rat, and Pig Anti-Bcl-X Antibody Product Attributes

Species: Human, Mouse, Rat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Bcl-X Antibody Clone: 2H12
Clone 2H12 Host and Isotype: Mouse IgG2a
Anti-Human, Mouse, Rat, and Pig Bcl-X Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
Cellular Localization of Antibody Cytoplasmic, cell/nuclear membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Bcl-X Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-BCL2L1 antibody staining is expected to be primarily localized to the mitochondria.

Observed Antibody Staining Data By Tissue Type:

Variations in Bcl-X antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Bcl-X could be seen in cells in the granular layer in cerebellum, chondrocytes in mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the colon, duodenum, endometrium, epididymis, rectum, salivary gland and thyroid gland, hepatocytes in liver, lymphoid tissue in appendix, myocytes in skeletal muscle, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, non-germinal center cells in the tonsil, peripheral nerve/ganglion in colon, pneumocytes in lung, Purkinje cells in the cerebellum and squamous epithelial cells in the esophagus. We were unable to detect Bcl-X in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-X expression as measured by anti-Bcl-X antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + - + - ++ + + + - + + ++ - + - + + + +
BCL2L1 Variability ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ + ++ ++ +++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Bcl-X Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Bcl-X Antibody Clone 2H12

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