Human, Mouse, Rat, and Pig Anti-Bcl-X Antibody Product Attributes
Species: Human, Mouse, Rat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Bcl-X Antibody Clone: BX006 + 2H12
Clone BX006 + 2H12 Host and Isotype: Mouse IgG
Anti-Human, Mouse, Rat, and Pig Bcl-X Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.
Cellular Localization of Antibody Cytoplasmic, cell/nuclear membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Bcl-X Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-BCL2L1 antibody staining is expected to be primarily localized to the mitochondria.Observed Antibody Staining Data By Tissue Type:
Variations in Bcl-X antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Bcl-X could be seen in cells in the granular layer in cerebellum, chondrocytes in mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the colon, duodenum, endometrium, epididymis, rectum, salivary gland and thyroid gland, hepatocytes in liver, lymphoid tissue in appendix, myocytes in skeletal muscle, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, non-germinal center cells in the tonsil, peripheral nerve/ganglion in colon, pneumocytes in lung, Purkinje cells in the cerebellum and squamous epithelial cells in the esophagus. We were unable to detect Bcl-X in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-X expression as measured by anti-Bcl-X antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | + | - | + | - | ++ | + | + | + | - | + | + | ++ | - | + | - | + | + | + | + |
| BCL2L1 Variability | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | ++ | +++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's Bcl-X Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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