Human Anti-BCL10 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-BCL10 Antibody Clone: SPM520
Clone SPM520 Host and Isotype: Mouse IgG1 kappa
Anti-Human BCL10 Positive Control Sample: WEHI-231 or Ramos cells or lymphoma.
Cellular Localization of Antibody Nuclear, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human BCL10 recombinant protein (epitope aa122-168)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
BCL10 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-BCL10 antibody staining is expected to be primarily localized to the nucleoplasm.Observed Antibody Staining Data By Tissue Type:
Variations in BCL10 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of BCL10 could be seen in cells in the seminiferous ducts in testis, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the epididymis, prostate, salivary gland, stomach and thyroid gland, melanocytes in skin, neuronal cells in the cerebral cortex, squamous epithelial cells in the cervix and uterine, esophagus, oral mucosa, tonsil and vagina. We were unable to detect BCL10 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of BCL10 expression as measured by anti-BCL10 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | + | - | + | ++ | + | - | + | - | + | +++ | + | + | + | + | - | ++ | ++ | - | + | + |
BCL10 Variability | ++ | ++ | ++ | ++ | ++ | + | + | ++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's BCL10 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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