FFPE human melanoma sections stained with 100 ul anti-Beta-2 Microglobulin (clone B2M/961) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.FFPE human renal cell carcinoma sections stained with 100 ul anti-Beta-2 Microglobulin (clone B2M/961) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-Beta-2 Microglobulin antibody (Clone B2M/961). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Formalin-fixed, paraffin-embedded human Melanoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).

Anti-Beta-2 Microglobulin Antibody Clone B2M/961

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 567-MSM5
Species: Human and non-human primates
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
567-MSM5-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 567-MSM5-P1
Beta-2 Microglobulin General Information
Alternate Names
?2 microglobulin, B2M, B2 microglobulin
Molecular Weight
12kDa
Chromosomal Location
15q21-q22.2
Curated Database and Bioinformatic Data
Gene SymbolB2M
Entrez Gene ID567
Ensemble Gene IDENSG00000166710
RefSeq Protein Accession(s)NP_004039, XP_005254606
RefSeq mRNA Accession(s)NM_004048, XM_005254549,
RefSeq Genomic Accession(s)NC_018926, NC_000015, NG_187605, NG_012920
UniProt ID(s)Q9UDF4, P01884, P61769
UniGene ID(s)Q9UDF4, P01884, P61769
HGNC ID(s)914
Cosmic ID(s)B2M
KEGG Gene ID(s)hsa:567
PharmGKB ID(s)PA25207
General Description of Beta-2 Microglobulin.
Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3), a non-covalent associating light chain, known as beta-2-Microglobulin. beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain, forms an immunoglobulin domain-like structure that mediates proper folding, expression of MHC class 1 molecules. The alpha1, alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Human and non-human primates Anti-Beta-2 Microglobulin Antibody Product Attributes

Species: Human and non-human primates
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Beta-2 Microglobulin Antibody Clone: B2M/961
Clone B2M/961 Host and Isotype: Mouse IgG2b kappa
Anti-Human and non-human primates Beta-2 Microglobulin Positive Control Sample: HL-60 or HeLa cells. Melanomas, Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.
Cellular Localization of Antibody Cell Surface, Extracellular (Secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Full length recombinant human B2M protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Beta-2 Microglobulin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-B2M antibody staining is expected to be primarily localized to the cytosol, golgi apparatus and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Beta-2 Microglobulin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow, macrophages in lung, non-germinal center cells in the lymph node and tonsil, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and squamous epithelial cells in the tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow, macrophages in lung, non-germinal center cells in the lymph node and tonsil, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and squamous epithelial cells in the tonsil. Low, but measureable presence of Beta-2 Microglobulin could be seen inadipocytes in breast and mesenchymal tissue, bile duct cells in the liver, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, chondrocytes in mesenchymal tissue, exocrine glandular cells in the pancreas, fibroblasts in skin and mesenchymal tissue, glandular cells in the breast and parathyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hepatocytes in liver, myocytes in heart muscle and skeletal muscle, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue, peripheral nerve/ganglion in colon, Purkinje cells in the cerebellum, smooth muscle cells in the smooth muscle, squamous epithelial cells in the cervix and uterine and vagina. We were unable to detect Beta-2 Microglobulin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Beta-2 Microglobulin expression as measured by anti-Beta-2 Microglobulin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - + ++ + - + ++ - ++ + + + + + - ++ - ++ +
B2M Variability +++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ +++

Limitations and Warranty

enQuire Bio's Beta-2 Microglobulin Anti-Human, Primate Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Beta-2 Microglobulin Antibody Clone B2M/961

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