Human Anti-BRCA1 Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-BRCA1 Antibody Clone: BRCA1/1398
Clone BRCA1/1398 Host and Isotype: Mouse IgG1
Anti-Human BRCA1 Positive Control Sample: A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
Cellular Localization of Antibody Cytoplasmic and Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human BRCA1 protein fragment (aa445-620)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
BRCA1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in BRCA1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin and germinal center cells in the lymph node. More moderate antibody staining intensity was present in epidermal cells in the skin and germinal center cells in the lymph node. Low, but measureable presence of BRCA1 could be seen inadipocytes in breast and mesenchymal tissue, bile duct cells in the liver, cells in the endometrial stroma in endometrium, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, chondrocytes in mesenchymal tissue, endothelial cells in the colon, fibroblasts in mesenchymal tissue, glandular cells in the salivary gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hepatocytes in liver, myocytes in heart muscle and skeletal muscle, neuronal cells in the hippocampus, neuropil in cerebral cortex, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue, peripheral nerve/ganglion in colon and smooth muscle cells in the smooth muscle. We were unable to detect BRCA1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of BRCA1 expression as measured by anti-BRCA1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | + | ++ | ++ | - | ++ | ++ | + | - | + | ++ | + | + | + | - | + | + | ++ | ++ | ++ |
| BRCA1 Variability | +++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | +++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's BRCA1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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