Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with c-Myc Monoclonal Antibody (MYC275).

Anti-c-Myc Oncoprotein Antibody Clone MYC275

$ 298.00
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SKU: 4609-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4609-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4609-MSM3-P1
c-Myc Oncoprotein General Information
Alternate Names
Myc, c-Myc, cMyc
Molecular Weight
62-64kDa
Chromosomal Location
8q24.21
Curated Database and Bioinformatic Data
Gene SymbolMYC
Entrez Gene ID4609
Ensemble Gene IDENSG00000136997
RefSeq Protein Accession(s)NP_001341799, NP_002458
RefSeq mRNA Accession(s)NM_001354870, NM_002467
RefSeq Genomic Accession(s)NC_000008, NG_007161, NC_018919
UniProt ID(s)P01106, A0A087WVR4
UniGene ID(s)P01106, A0A087WVR4
HGNC ID(s)7553
Cosmic ID(s)MYC
KEGG Gene ID(s)hsa:4609
PharmGKB ID(s)PA31353
General Description of c-Myc Oncoprotein.
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells, plays an important role in cellular proliferation, differentiation, apoptosis, cell cycle progression. The phosphorylation of c-Myc has been investigated, previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2, C-Jun N terminal Kinase (JNK) in cell proliferation, cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis, angiogenesis that distribute blood throughout the cells,, which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Human Anti-c-Myc Oncoprotein Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-c-Myc Oncoprotein Antibody Clone: MYC275
Clone MYC275 Host and Isotype: Mouse IgG1 kappa
Anti-Human c-Myc Oncoprotein Positive Control Sample: HL-60 cells or breast carcinoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human c-myc protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

c-Myc Oncoprotein Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MYC antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in c-Myc Oncoprotein antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, Purkinje cells in the cerebellum, squamous epithelial cells in the cervix, uterine, neuronal cells in the hippocampus, pneumocytes in lung and cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, Purkinje cells in the cerebellum, squamous epithelial cells in the cervix, uterine, neuronal cells in the hippocampus, pneumocytes in lung and cells in the seminiferous ducts in testis. Low, but measureable presence of c-Myc Oncoprotein could be seen inhematopoietic cells in the bone marrow, glandular cells in the cervix, uterine, endothelial cells in the colon, glandular cells in the colon, endometrium and fallopian tube, glial cells in the hippocampus, bile duct cells in the liver, germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the parathyroid gland, trophoblastic cells in the placenta, glandular cells in the salivary gland, fibroblasts in skin, Langerhans in skin, melanocytes in skin, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the white pulp in spleen and glandular cells in the thyroid gland. We were unable to detect c-Myc Oncoprotein in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of c-Myc Oncoprotein expression as measured by anti-c-Myc Oncoprotein antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - ++ ++ + - - ++ ++ - + ++ ++ ++ ++ + ++ + - ++
MYC Variability ++ ++ ++ ++ + ++ ++ + ++ ++ +++ ++ ++ +++ ++ ++ ++ ++ ++ +

Limitations and Warranty

enQuire Bio's c-Myc Oncoprotein Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-c-Myc Oncoprotein Antibody Clone MYC275

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