Immunohistochemical staining of CA 15-3  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

CA 15-3 [DBMM1] Antibody (cGMP).

$ 128.00$ 598.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD77
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

Product NumberDescriptionPrice
QIVD77-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD77-0.5ml Size: 0.5 ml, Format: Concentrate $ 368.00
QIVD77-1ml Size: 1 ml, Format: Concentrate $ 598.00
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Datasheets and Documentation
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SKU: QIVD77-0.1ml
CA 15-3 General Information
Alternate Names
Molecular Weight
122.1 kDa
Chromosomal Location
q22 [chr: 1] [chr_start: 155185824] [chr_end: 155192916] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolMUC1
Entrez Gene ID4582
RefSeq Protein Accession(s)NP_001018016; NP_001018017; NP_001037856; NP_001191215; NP_001191218; NP_001191221; NP_001037857; NP_001191217; NP_001037855; NP_001191219; NP_001191220; NP_001191222; NP_002447; NP_001191216; NP_001037858; NP_001191214; NP_001191225; NP_001191223; NP_001191224; NP_001191226
RefSeq mRNA Accession(s)NM_001044392; NM_001204292; NM_002456; NM_001018021; NM_182741; NM_001204286; NM_001204293; NM_001204294; NM_001044390; NM_001044393; NM_001044391; NM_001204291; NM_001204295; NM_001018016; NM_001204285; NM_001204287; NM_001204290; NM_001204297; NM_001018017; NM_001204288; NM_001204289; NM_001204296
RefSeq Genomic Accession(s)NC_000001; NG_029383;
UniProt ID(s)P15941
PharmGKB ID(s)PA31309
KEGG Gene ID(s)hsa:4582
Associated Diseases (KEGG IDs)MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes. {ECO:0000269|PubMed:20816948}.; Medullary cystic kidney disease 1 (MCKD1) [MIM:174000]: A form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade. {ECO:0000269|PubMed:23396133}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of CA 15-3 .
This antibody is specific to epithelial membrane antigen (EMA), CA 15-3, or polymorphic epithelial mucin. This antibody stains an underglycosylated MUC1 often present on carcinoma cells.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:10-1:20
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CA 15-3 Antibody Clone: DBMM1
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Breast carcinoma
Cellular Localization of Antibody DBMM1 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Human breast cancer cell line ZR-75-1
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CA 15-3 Information for Pathologists

Summary:

Also called CD227, MUC1 and episialin. Large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cells. Pathophysiology Normally acts as barrier to apical surface of epithelial cells, playing a protective and regulatory role. Inhibits formation of E-cadherin / beta catenin complex.

Notable Clinical Features:

Highly expressed by most adenocarcinomas, associated with poor prognosis (Hum Pathol 1995;26:432). In breast cancer, aberrant cytoplasmic and membranous localization (associated with micropapillary subtype) is associated with poor outcome (Mod Pathol 2005;18:1295). Shed into the bloodstream of adenocarcinoma patients, used in commercial serum tumor marker assays (CA15-3). Uses by pathologists Common epithelial marker.

Common Uses By Pathologists:

Common epithelial marker. Marker of meningioma. Marker of Paget disease (Am J Surg Pathol 2001;25:1469). In breast and bladder micropapillary carcinoma, MUC1 stains stroma-facing surface of cell clusters (basal), which accentuates outlines of micropapillary units to form a distinct band on this surface. Distinguish systemic anaplastic large cell lymphoma (MUC1+) from cutaneous anaplastic large cell lymphoma (usually MUC1-, Am J Surg Pathol 2008;32:1421).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CA 15-3 [DBMM1] Antibody (cGMP).