Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:50-1:100|
|Pretreatment||EDTA Buffer pH 8.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Caldesmon Antibody Clone: hHCD
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Endometrial cancer
Cellular Localization of Antibody hHCD Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with human uterus smooth muscle extract.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Caldesmon Information for Pathologists
Summary:Caldesmon binds calcium, calmodulin, tropomyosin and actin and regulates smooth muscle contraction (Int J Biochem Cell Biol 1997;29:1047). Regulates smooth muscle contraction and relaxation. May play a role in preventing unwanted contractions (Am J Physiol Heart Circ Physiol 2009;297:H1930). Interpretation: cytoplasmic staining. h-caldesmon is high molecular weight isoform, found in smooth muscle cells; l-caldesmon isoform is distributed in non-muscle cells (J Biol Chem 1991;266:355).
Common Uses By Pathologists:Very useful to distinguish smooth muscle cells / lesions (positive) from myofibroblastic cells / lesions (negative or only a few cells positive, Am J Clin Pathol 2000;114:746, Am J Dermatopathol 2006;28:105). Distinguish uterine leiomyoma or leiomyosarcoma (positive) from endometrial stromal tumors (negative, Am J Surg Pathol 2001;25:455), but endometrial stromal tumors may have focal smooth muscle differentiation (Am J Surg Pathol 2002;26:1142). Sensitive and specific for epithelioid peritoneal mesothelioma (positive) versus ovarian serous carcinoma (negative, Am J Surg Pathol 2007;31:1139) and epithelioid pleural mesothelioma (positive) versus pulmonary adenocarcinoma (negative, Am J Surg Pathol 2006;30:463). Microscopic (histologic) images Images hosted on other servers:.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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