Formalin-fixed, paraffin embedded human uterus sections stained with 100 ul anti-Caldesmon, HMW (clone CALD1/820) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.

Anti-Caldesmon, HMW Antibody Clone CALD1/820

Name: Anti-Caldesmon, HMW Antibody Clone CALD1/820
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 800-MSM2
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
800-MSM2-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 800-MSM2-P1

Caldesmon, HMW General Information
Alternate Names
Caldesmon, CALD1
Molecular Weight
150kDa
Chromosomal Location
7q33
Curated Database and Bioinformatic Data
Gene Symbol	CALD1
Entrez Gene ID	800
Ensemble Gene ID	ENSG00000122786
RefSeq Protein Accession(s)	XP_016868141, XP_016868142, NP_149130, XP_016868139, XP_016868140, XP_016868143, NP_149129, NP_149131, NP_149347, NP_004333
RefSeq mRNA Accession(s)	XR_001744877, XR_927535, XR_927537, NM_004342, NM_033140, XR_001744880, XM_017012650, XM_017012654, XR_927541 XR_001744878, XM_017012651, XM_017012652, XR_001744879, XM_017012653, XR_001744881, XR_927542, NM_033138, NM_033139, NM_033157
RefSeq Genomic Accession(s)	NC_000007, NG_029186, NC_018918
UniProt ID(s)	A0A140VKA0, Q05682
UniGene ID(s)	A0A140VKA0, Q05682
HGNC ID(s)	1441
Cosmic ID(s)	CALD1
KEGG Gene ID(s)	hsa:800
PharmGKB ID(s)	PA26034
General Description of Caldesmon, HMW.
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility, cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue, cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts, is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle, non-muscle contraction.

Human and Rat (-) Anti-Caldesmon, HMW Antibody Product Attributes

Species: Human and Rat (-)

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Caldesmon, HMW Antibody Clone: CALD1/820

Clone CALD1/820 Host and Isotype: Mouse IgG1 kappa

Anti-Human and Rat (-) Caldesmon, HMW Positive Control Sample: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant full-length human CALD1 protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Caldesmon, HMW Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CALD1 antibody staining is expected to be primarily localized to the actin filaments and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Caldesmon, HMW antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, decidual cells in the placenta, ovarian stroma cells in the ovary and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, decidual cells in the placenta, ovarian stroma cells in the ovary and smooth muscle cells in the smooth muscle. Low, but measureable presence of Caldesmon, HMW could be seen inadipocytes in breast, cells in the seminiferous ducts in testis, cells in the tubules in kidney, fibroblasts in mesenchymal tissue, glandular cells in the breast, endometrium, epididymis, prostate, salivary gland and stomach, hepatocytes in liver, islets of Langerhans in pancreas, Leydig cells in the testis, macrophages in lung, pneumocytes in lung, squamous epithelial cells in the cervix and uterine, oral mucosa and tonsil. We were unable to detect Caldesmon, HMW in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Caldesmon, HMW Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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