Human Anti-Calgranulin B Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Calgranulin B Antibody Clone: CAGB/426
Clone CAGB/426 Host and Isotype: Mouse IgM kappa
Anti-Human Calgranulin B Positive Control Sample: Tonsil, lymph node, or spleen
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CAGB protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Calgranulin B Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-S100A9 antibody staining is expected to be primarily localized to the cell junctions and cytosol.Observed Antibody Staining Data By Tissue Type:
Variations in Calgranulin B antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of Calgranulin B could be seen in cells in the white pulp in spleen. We were unable to detect Calgranulin B in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Calgranulin B expression as measured by anti-Calgranulin B antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | + | - | + | - | + | - | ++ | - | - | - | - | - | - | ++ | - | - | - | - |
S100A9 Variability | ++ | + | ++ | + | ++ | + | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | + | + | + | ++ |
Limitations and Warranty
enQuire Bio's Calgranulin B Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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