Human and Rat Anti-Calponin-1 Antibody Product Attributes
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-Calponin-1 Antibody Clone: CALP
Clone CALP Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat Calponin-1 Positive Control Sample: Myoepithelial cells in breast ducts
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Crude human uterus extract
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Calponin-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CNN1 antibody staining is expected to be primarily localized to the actin filaments. There is variability in either the signal strength or the localization of signal in actin filaments from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in Calponin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in smooth muscle cells in the smooth muscle. Low, but measureable presence of Calponin-1 could be seen in. We were unable to detect Calponin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Calponin-1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.