Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Monoclonal Antibody (CALP).
Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Monoclonal Antibody (CALP).

Anti-Calponin-1 Antibody Clone CALP

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1264-MSM1
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1264-MSM1-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 1264-MSM1-P1
Calponin-1 General Information
Alternate Names
Calponin 1, CNN1
Molecular Weight
34kDa
Chromosomal Location
19p13.2
Curated Database and Bioinformatic Data
Gene SymbolCNN1
Entrez Gene ID1264
Ensemble Gene IDENSG00000130176
RefSeq Protein Accession(s)NP_001290, NP_001295270, XP_016881778, NP_001295271, XP_005259798
RefSeq mRNA Accession(s)XM_005259741, NM_001299, NM_001308342, NM_001308341, XM_017026289
RefSeq Genomic Accession(s)NC_000019, NC_018930
UniProt ID(s)Q53FP8, P51911, V9HWA5, B7Z7E1
UniGene ID(s)Q53FP8, P51911, V9HWA5, B7Z7E1
HGNC ID(s)2155
Cosmic ID(s)CNN1
KEGG Gene ID(s)hsa:1264
PharmGKB ID(s)PA26665
General Description of Calponin-1.
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form, a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular, visceral smooth muscle. In Western Blot, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle, is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin, tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells, has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Human and Rat Anti-Calponin-1 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Calponin-1 Antibody Clone: CALP
Clone CALP Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat Calponin-1 Positive Control Sample: Myoepithelial cells in breast ducts
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Crude human uterus extract
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Calponin-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CNN1 antibody staining is expected to be primarily localized to the actin filaments. There is variability in either the signal strength or the localization of signal in actin filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Calponin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in smooth muscle cells in the smooth muscle. Low, but measureable presence of Calponin-1 could be seen in. We were unable to detect Calponin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Calponin-1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Calponin-1 Antibody Clone CALP”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-Calponin-1 Antibody Clone CALP

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-1264-MSM1-P1-anti-Calponin-1-antibody-300x225.jpeg