Formalin-fixed, paraffin embedded human uterus sections stained with 100 ul anti-Calponin-1 (clone rCNN1/832) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.
Formalin-fixed, paraffin embedded human uterus sections stained with 100 ul anti-Calponin-1 (clone rCNN1/832) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Tris 1mM EDTA, pH 9.0.Data from SDS-PAGE analysis of Anti-Calponin-1 antibody (Clone rCNN1/832). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832).

Anti-Calponin-1 Antibody Clone rCNN1/832

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1264-MSM6
Species: Human and Rat
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1264-MSM6-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
1264-MSM6-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
1264-MSM6-P2 Size: 200 ug, Tag: Unconjugated $ 518.00
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SKU: 1264-MSM6-P0
Calponin-1 General Information
Alternate Names
Calponin 1, CNN1
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolCNN1
Entrez Gene ID1264
Ensemble Gene IDENSG00000130176
RefSeq Protein Accession(s)NP_001290, NP_001295270, XP_016881778, NP_001295271, XP_005259798
RefSeq mRNA Accession(s)XM_005259741, NM_001299, NM_001308342, NM_001308341, XM_017026289
RefSeq Genomic Accession(s)NC_000019, NC_018930
UniProt ID(s)Q53FP8, P51911, V9HWA5, B7Z7E1
UniGene ID(s)Q53FP8, P51911, V9HWA5, B7Z7E1
HGNC ID(s)2155
Cosmic ID(s)CNN1
KEGG Gene ID(s)hsa:1264
PharmGKB ID(s)PA26665
General Description of Calponin-1.
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western Blot, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Human and Rat Anti-Calponin-1 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Immunohistochemistry (IHC).
Clonality: Recombinant Monoclonal
Anti-Calponin-1 Antibody Clone: rCNN1/832
Clone rCNN1/832 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat Calponin-1 Positive Control Sample: Myoepithelial cells in breast ducts
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with or without 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CNN1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Calponin-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CNN1 antibody staining is expected to be primarily localized to the actin filaments. There is variability in either the signal strength or the localization of signal in actin filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Calponin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in smooth muscle cells in the smooth muscle. Low, but measureable presence of Calponin-1 could be seen in. We were unable to detect Calponin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Calponin-1 Recombinant Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Calponin-1 Antibody Clone rCNN1/832 IHC human Uterus (rCNN1 832)-300x225.jpg 832) Gel.jpg endometrium calponin-1 (rCNN1 832)-133x300.jpg