Formalin-fixed, paraffin-embedded human Tonsil stained with Calprotectin Monoclonal Antibody (CPT/128)

Anti-Calprotectin Antibody Clone CPT/1028

Name: Anti-Calprotectin Antibody Clone CPT/1028
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 6280-MSM4
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
6280-MSM4-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 6280-MSM4-P1

Calprotectin General Information
Alternate Names
S100 calcium-binding protein A9, S100A9, migration inhibitory factor-related protein 14, MRP14, calgranulin B, S100A9
Molecular Weight
14kDa
Chromosomal Location
1q21
Curated Database and Bioinformatic Data
Gene Symbol	S100A9
Entrez Gene ID	6280
Ensemble Gene ID	ENSG00000163220
RefSeq Protein Accession(s)	NP_002956
RefSeq mRNA Accession(s)	NM_002965
RefSeq Genomic Accession(s)	NC_018912, NC_000001
UniProt ID(s)	P06702
UniGene ID(s)	P06702
HGNC ID(s)	10499
Cosmic ID(s)	S100A9
KEGG Gene ID(s)	hsa:6280
PharmGKB ID(s)	PA34911
General Description of Calprotectin.
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain, a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes, is also found in monocytes, macrophages, ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra-, intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating, antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools,, synovial fluid during infection, inflammatory conditions.This MAb reacts with neutrophils, monocytes, macrophages,, squamous mucosal epithelia, is important for identifying macrophages in tissue sections.

Human Anti-Calprotectin Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Calprotectin Antibody Clone: CPT/1028

Clone CPT/1028 Host and Isotype: Mouse IgM kappa

Anti-Human Calprotectin Positive Control Sample: Tonsil, lymph node, or spleen

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant human Calprotectin protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Calprotectin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-S100A9 antibody staining is expected to be primarily localized to the cell junctions and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Calprotectin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of Calprotectin could be seen in cells in the white pulp in spleen. We were unable to detect Calprotectin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Calprotectin expression as measured by anti-Calprotectin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	+	-	+	-	+	-	++	-	-	-	-	-	-	++	-	-	-	-
S100A9 Variability	++	+	++	+	++	+	++	++	++	++	+	++	++	++	+	++	+	+	+	++

Limitations and Warranty

enQuire Bio's Calprotectin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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