Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-Calprotectin (clone SPM281) at 1:400. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-Calprotectin (clone SPM281) at 1:400. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Anti-Calprotectin Antibody Clone SPM281

$ 488.00
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SKU: 6279-MSM1X
Species: Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6279-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 6279-MSM1X-P1
Calprotectin General Information
Alternate Names
S100A8, S100A9 Heterodimer, Calprotectin
Molecular Weight
12-14kDa (doublet)
Chromosomal Location
1q21
Curated Database and Bioinformatic Data
Gene SymbolS100A8 & S100A9
Entrez Gene ID, 6280
Ensemble Gene ID, ENSG00000163220
RefSeq Protein Accession(s), NP_002956
RefSeq mRNA Accession(s), NM_002965
RefSeq Genomic Accession(s), NC_018912, NC_000001
UniProt ID(s), P06702
UniGene ID(s), P06702
HGNC ID(s), 10499
Cosmic ID(s), S100A9
KEGG Gene ID(s), hsa:6280
PharmGKB ID(s), PA34911
General Description of Calprotectin.
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain, a 14kDa beta chain expressed by granulocytes, monocytes, by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal, inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffers cells), lung (alveolar macrophages), lymph nodes (free, fixed macrophages), spleen (free, fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural, peritoneal macrophages), skin (histiocytes, Langerhans's cell), in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing, destroying many potential pathogens including bacteria, pathogenic protozoa, fungi, helminthes. This MAb reacts with neutrophils, monocytes, macrophages,, squamous mucosal epithelia, has been shown as an important marker for identifying macrophages in tissue sections.

Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse Anti-Calprotectin Antibody Product Attributes

Species: Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Calprotectin Antibody Clone: SPM281
Clone SPM281 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse Calprotectin Positive Control Sample: Tonsil, lymph node, or spleen
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Affinity purified monocyte membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Calprotectin Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's Calprotectin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Calprotectin Antibody Clone SPM281

https://enquirebio.com/wp-content/uploads/2019/01/Calprotectin IHC human Tonsil (SPM281)-300x225.jpg