Discontinued
Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Clonality: Monoclonal
Anti-Calretinin Antibody Clone: SP13
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Mesothelioma
Cellular Localization of Antibody SP13 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant full-length mouse calretinin protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Calretinin Information for Pathologists
Summary:
Calcium binding protein structurally related to S100 and inhibin. Interpretation Nuclear and cytoplasmic staining. Uses by pathologists Differentiate (as part of a panel) epithelioid pleural mesothelioma (positive) from lung adenocarcinoma (negative, Am J Surg Pathol 2003;27:1031).Common Uses By Pathologists:
Differentiate (as part of a panel) epithelioid pleural mesothelioma (positive) from lung adenocarcinoma (negative, Am J Surg Pathol 2003;27:1031). Differentiate (as part of a panel) epithelioid peritoneal mesothelioma (positive) from ovarian serous papillary carcinoma (usually negative, Am J Surg Pathol 2007;31:1139). Differentiate reactive mesothelial cells (positive) from carcinomas (negative) in effusion cytology (Am J Clin Pathol 2001;116:709, Cytopathology 2008;19:218), ascites fluid / peritoneal lavage (Tohoku J Exp Med 2005;206:31) or pleural biopsies (Am J Surg Pathol 2007;31:914). Differentiate (as part of a panel) mesothelioma (positive) from metastatic renal cell carcinoma (negative, Histopathology 2002;41:301). Differentiate (as part of a panel) adrenal cortical lesions (calretinin+) from metastatic clear cell renal cell carcinoma (negative, Am J Surg Pathol 2011;35:678).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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