Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CEA Monoclonal Antibody (C66/13)
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CEA Monoclonal Antibody (C66/13)

Anti-Carcinoembryonic Antigen / CD66 Antibody Clone C66/1030

$ 329.00
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SKU: 1048-MSM8
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1048-MSM8-P1
Carcinoembryonic Antigen / CD66 General Information
Alternate Names
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, anti-Carcinoembryonic Antigen-related Cell Adhesion Molecule 5 antibody, anti-CEACAM5 antibody, anti-CD66 antibody
Molecular Weight
80-200kDa
Chromosomal Location
19q13.1-19q13.2
Curated Database and Bioinformatic Data
Gene SymbolCEACAM1 & CEACAM5
Entrez Gene ID1048, 634
Ensemble Gene IDENSG00000105388, ENSG00000079385
RefSeq Protein Accession(s)XP_016881634, XP_016881635, NP_001278413, NP_004354, XP_005258470, NP_001295327, XP_011524624, NP_001020083, NP_001192273, NP_001171744, NP_001703, NP_001171742, NP_001171745, XP_011525508
RefSeq mRNA Accession(s)XM_017026145, XM_017026146, XM_011526322, NM_001291484, XM_005258413, NM_001308398, NM_004363, NM_001184815, NM_001184816, NM_001205344, NM_001024912, XM_011527206 NM_001184813, NM_001712
RefSeq Genomic Accession(s)NC_000019, NC_018930NC_018930, NC_000019, NG_029051
UniProt ID(s)P06731, Q53G30, A0A024R0K5, 13688, Q3KRG8
UniGene ID(s)P06731, Q53G30, A0A024R0K5, 13688, Q3KRG8
HGNC ID(s)1817, 1814
Cosmic ID(s)CEACAM5, CEACAM1
KEGG Gene ID(s)hsa:1048, hsa:634
PharmGKB ID(s)PA26361, PA26358
General Description of Carcinoembryonic Antigen / CD66.
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut, is re-expressed in increased amounts in intestinal carcinomas, several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA), with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues, is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma, in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary,, endocervix.

Human Anti-Carcinoembryonic Antigen / CD66 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Carcinoembryonic Antigen / CD66 Antibody Clone: C66/1030
Clone C66/1030 Host and Isotype: Mouse IgG1 kappa
Anti-Human Carcinoembryonic Antigen / CD66 Positive Control Sample: MCF7 or 293T cells. Colon carcinoma
Cellular Localization of Antibody Cytoplasmic, luminal surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CEA protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Carcinoembryonic Antigen / CD66 Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's Carcinoembryonic Antigen / CD66 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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